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Loss-of-function analysis of Rab GTPases involved in the early stages of biosynthetic transport in arabidposis
Reference
BBS/B/03904
Principal Investigator / Supervisor
Professor Ian Moore
Co-Investigators /
Co-Supervisors
Institution
University of Oxford
Department
Plant Sciences
Funding type
Research
Value (£)
182,949
Status
Completed
Type
Research Grant
Start date
05/05/2004
End date
04/05/2007
Duration
36 months
Abstract
The use of dominant negative mutants in transient expression assays has produced evidence that the 4 Rabl-related GTPases in Arabidopsis can be subdivided into two families that act on membrane traffic by interaction with different molecules. We will use 2 loss-of-function approaches, insertional mutagenesis and chemically-inducible siRNA, to establish the redundancy relationship within the RabD branch. We will analyse diverse fluorescent protein markers of secretion to ask whether the two branches of the RabD group have different loss-of-function phenotypes and whether dominant-negative and loss-of-function phenotypes are similar. We will also use loss of function phenotypes to test conflicting hypotheses based on the analysis of dominant-negative mutants regarding the role of Rab2 homologues in plants.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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