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The cyclin-CDK complexes of arabidopsis

Reference	BBS/B/03815
Principal Investigator / Supervisor	Professor James Murray
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Institute of Biotechnology
Funding type	Research
Value (£)	361,769
Status	Completed
Type	Research Grant
Start date	01/08/2004
End date	31/05/2009
Duration	58 months

Abstract

The cell cycle is regulated by complexes of cyclin-dependent kinases (CDKs) and cyclins. Plants contain much larger numbers of these key cell cycle regulators than other groups of organisms, and possess a unique class of CDK. Arabidopsis has a total of 5 CDK genes and 29 cyclins. Very little is known about the nature of the complexes that are formed. We have shown that bulk CDK-cyclin complexes can be isolated from cell suspension cultures and components and potential substrates identified by mass spectrometry using a synchronisable cell suspension culture. Here we propose to identify individual CDK and cyclin complexes by tandem affinity purification, analyse their components and confirm their intracellular location by GFP tagging.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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