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Analysis of the mechanisms of pre-mRNA splicing by single molecule fluorescence

Reference	BBS/B/0367X
Principal Investigator / Supervisor	Professor Ian EPERON
Co-Investigators / Co-Supervisors	Professor Clive Bagshaw
Institution	University of Leicester
Department	Biochemistry
Funding type	Research
Value (£)	277,169
Status	Completed
Type	Research Grant
Start date	18/10/2004
End date	17/10/2007
Duration	36 months

Abstract

Pre-mRNA splicing is highly complex. Over a hundred proteins and several RNA species assemble into dynamic complexes, in a pathway defined by several stable partial complexes. However, the relevance of these has been challenged and very little is known about transient or unstable components. Even less is known about the mechanisms by which many proteins affect the selection of splice sites. Splicing reactions in vitro cannot be reconstituted from pure components but require nuclear extracts. We will use single molecule fluorescence to follow the order, stoichiometry and rates of assembly and disassembly of components during splicing reactions. This methodology may have widespread uses for other complex processes.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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