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Substrate-specificity of protein import pathways in chloroplasts
Reference
BBS/B/03629
Principal Investigator / Supervisor
Professor Paul Jarvis
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Biology
Funding type
Research
Value (£)
242,092
Status
Completed
Type
Research Grant
Start date
01/07/2004
End date
30/06/2007
Duration
36 months
Abstract
The chloroplast protein import receptors, Toc159 and Toc34, are represented in Arabidopsis by four and two homologues, respectively, which are proposed to have different preprotein recognition specificities. Arabidopsis knockout mutants for each homologue will be used to investigate their involvement in substrate-specific import pathways: (1) Double mutant and transgenic complementation studies will assess functional relationships between the proteins; (2) Transcriptomics will compare the gene expression profiles of each mutant; (3) Proteomics will compare the protein complements of wild-type and mutant chloroplasts; (4) In vitro import assays will measure import rates for selected preproteins in the mutants; (5) Fluorescence microscopy and preprotein-GFP fusions will beused to study the protein defects of the mutants in vivo.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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