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A physical and biochemical study of the S. cerevisiae actomyosin contractile ring

Reference	BBS/B/03289
Principal Investigator / Supervisor	Dr Clive Price
Co-Investigators / Co-Supervisors	Professor Michael Stark
Institution	Lancaster University
Department	Biological Sciences
Funding type	Research
Value (£)	262,533
Status	Completed
Type	Research Grant
Start date	01/08/2004
End date	31/07/2007
Duration	36 months

Abstract

Two related approaches will be used to physically characterise the contractile ring. First, affinity purification of epitope-tagged components of the contractile ring and the subsequent identification of the associated proteins by SDS-PAGE and MALDI-TOF mass spectrometry. Secondly, we have observed the release of the intact contractile ring from cells arrested in late mitosis, based upon this observation, we will develop a purification scheme for the entire structure. Subsequently a combination of genetic, biochemical and cell biology techniques will be employed to confirm the presence and function of newly identified proteins within the actomyosin ring.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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