Award details

Clock proteins and their interactions in the zebrafish circadian system

Reference	BBS/B/02886
Principal Investigator / Supervisor	Professor David Whitmore
Co-Investigators / Co-Supervisors
Institution	University College London
Department	Cell and Developmental Biology
Funding type	Research
Value (£)	335,211
Status	Completed
Type	Research Grant
Start date	06/08/2004
End date	05/08/2007
Duration	36 months

Abstract

Our understanding of the cellular circadian clock mechanism has progressed dramatically through the use of genetic approaches in Drosophila and mouse. However, as tissue is limiting in these systems, biochemical studies are often difficult to perform. This problem has been overcome in zebrafish, where we have produced several light responsive, clock-containing cell lines. These cells are a unique system for clock analysis, in that, for the first time, the amount of starting material is not limiting. The aim of this proposal is to employ large-scale proteomic approaches to analyse clock proteins, using antibodies to study their expression and localisation, and affinity purification to study the interactions and complexes they form.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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