Award details

Metabolic pathway promiscuity: a structural mechanistic and biocatalytic investigation

Reference	BBS/B/02746
Principal Investigator / Supervisor	Dr David Hough
Co-Investigators / Co-Supervisors	Dr Steven Bull, Professor Michael Danson
Institution	University of Bath
Department	Biology and Biochemistry
Funding type	Research
Value (£)	217,915
Status	Completed
Type	Research Grant
Start date	01/10/2004
End date	31/01/2008
Duration	40 months

Abstract

Glucose metabolism in the hyperthermophilic Archeaon, Sulfolobus solfataricus, involves a non-phoshorylating form of the Entner Doudoroff pathway. We have recently found that the three enzymes involved can accept a range of substrates, including glucose, galatose and xylose, and thus a single pathway can metabolise a range of substrates. This phenomenon, known as metabolic pathway promiscuity, has implications both for the physiology of the organism and for the evolution of metabolism, and in addition provides a series of enzymes that can be used as flexible scaffolds for biocatalytic applications. This project aims to define the structural basis of catalytic promiscuity of each enzyme, glucose dehydrogenase, gluconate dehydrase and KDG aldolase, to elucidate their detailed catalytic mechanisms, and to demonstrate their biocatalytic potential. (Joint with BBS/B/02789).

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BBS/B/02789 Metabolic pathway promiscuity: a structural mechanistic and biocatalytic investigation

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