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Metabolic pathway promiscuity: a structural mechanistic and biocatalytic investigation

ReferenceBBS/B/02746
Principal Investigator / Supervisor Dr David Hough
Co-Investigators /
Co-Supervisors
Dr Steven Bull, Professor Michael Danson
Institution University of Bath
DepartmentBiology and Biochemistry
Funding typeResearch
Value (£) 217,915
StatusCompleted
TypeResearch Grant
Start date 01/10/2004
End date 31/01/2008
Duration40 months

Abstract

Glucose metabolism in the hyperthermophilic Archeaon, Sulfolobus solfataricus, involves a non-phoshorylating form of the Entner Doudoroff pathway. We have recently found that the three enzymes involved can accept a range of substrates, including glucose, galatose and xylose, and thus a single pathway can metabolise a range of substrates. This phenomenon, known as metabolic pathway promiscuity, has implications both for the physiology of the organism and for the evolution of metabolism, and in addition provides a series of enzymes that can be used as flexible scaffolds for biocatalytic applications. This project aims to define the structural basis of catalytic promiscuity of each enzyme, glucose dehydrogenase, gluconate dehydrase and KDG aldolase, to elucidate their detailed catalytic mechanisms, and to demonstrate their biocatalytic potential. (Joint with BBS/B/02789).

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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