Award details

Defining the role of a nuclear/cytoplasmic shuttling protein involved in signal transduction by use of the proteomics

Reference	BBS/B/02681
Principal Investigator / Supervisor	Professor Anthony David Whetton
Co-Investigators / Co-Supervisors	Professor Simon James Gaskell
Institution	The University of Manchester
Department	Medical and Human Sciences
Funding type	Research
Value (£)	226,930
Status	Completed
Type	Research Grant
Start date	01/01/2005
End date	31/05/2008
Duration	41 months

Abstract

Fms Interacting Protein, FMIP, interacts with a receptor tyrosine kinase, Fms, at the plasma membrane but is found predominantly in the nucleus. Protein kinases C-mediated phosphorylation influences subcellular localisation. FMIP can suppress transactivation by a transcription factor, C/EBP, and the knockout mouse dies within 3 days of conception. Our FMIP monoclonal antibodies have enabled us to show that FMIP levels are regulated post-translationally, falling precipitously as multipotent hematopoietic cells commit to differentiation. Our aim is to characterise the binding partners of FMIP using a proteomic approach and assess phosphorylation effects on this process via site directed mutagenesis. We will also investigate FMIP proteolysis. Our objective is to understand the role of this novel protein more fully using proteomics.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Proteomics and Cell Function (PCF) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search