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The role centromere protein-F in chromosome segregation

Reference	BBS/B/02606
Principal Investigator / Supervisor	Professor Stephen Taylor
Co-Investigators / Co-Supervisors
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	217,823
Status	Completed
Type	Research Grant
Start date	01/06/2004
End date	31/05/2007
Duration	36 months

Abstract

Cenp-F is an unusual kinetochore protein in that it localises to the nuclear matrix in interphase, the nuclear envelope at the G2/M transition, is rapidly degraded after mitosis and is farnesylated. We have recently shown that farnesylation of Cenp-F is required for G2/M progression, its localisation to kinetochores, and its degradation. While the role of Cenp-F in mitosis remains elusive, preliminary observations using RNAi demonstrate that Cenp-F is indeed required for chromosome alignment and segregation. In this proposal we will have two independent approaches (RNAi and antibody injection) followed by time lapse microscopy to define the role of Cenp-F in mitosis. To elucidate the molecular mechanisms underlying the Cenp-F inhibition phenotype we will employ a number of molecular cell biology assays and perform a yeast two hybrid screen to identify novel interactors.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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