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High-throughput reverse genetics to determine legume gene function

Reference	BBS/B/02401
Principal Investigator / Supervisor	Professor Jonathan Jones
Co-Investigators / Co-Supervisors	Professor Trevor Wang
Institution	University of East Anglia
Department	Sainsbury Laboratory
Funding type	Research
Value (£)	326,238
Status	Completed
Type	Research Grant
Start date	01/06/2004
End date	30/09/2007
Duration	40 months

Abstract

We have established a TILLING reverse genetics tool in the model legume Lotus japonicus that enables the rapid identification of plants representing a series of mutant alleles in any gene of interest. We wish to capitalise on that investment and install a high-throughput pipeline to determine the function of several hundred genes concerned with legume development and metabolism. A major focus will be targeted on genes with potential roles in the establishment of the root symbioses and their carbon and nitrogen flow. Candidate genes have been identified through approaches such as transcriptional profiling and yeast two hybrid screens. In addition, we propose to identify mutants in genes implicated in other aspects of legume development via collaborations with the community. The phenotypes of mutants will be examined in detail both biochemically and cytologically.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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