Award details

Dairy niche adaptation by bovine mastitis pathogens: novel targets for control

Reference	BB/W014920/1
Principal Investigator / Supervisor	Professor Ross Fitzgerald
Co-Investigators / Co-Supervisors	Dr Michael Gutmann, Dr Nicola Lynskey, Dr Amy Pickering
Institution	University of Edinburgh
Department	The Roslin Institute
Funding type	Research
Value (£)	540,915
Status	Current
Type	Research Grant
Start date	05/09/2022
End date	04/09/2025
Duration	36 months

Abstract

Bovine mastitis is the most important disease affecting the global dairy industry. Mastitis can be caused by an array of different bacterial pathogens including Staphylococcus aureus and Streptococcus agalactiae. Infections can be subclinical or clinical and are often chronic in nature and difficult to treat due to production of biofilm, an intracellular tropism and antibiotic resistance. Our previous work has identified pathogenic clones of S. aureus specialized for intramammary infection of dairy cows, that have evolved via host-switching events from humans followed by adaptive evolution by gene acquisition and diversification. We have identified adaptive traits associated with the ability of bovine S. aureus and S. agalactiae strains to survive and proliferate in the dairy niche including enhanced growth associated with induction of curdling and the capacity to produce a novel biofilm in the presence of milk. Here, we will employ complementary approaches including population genomics, transcriptomics, transposon mutagenesis and proteomics to determine the genetic and functional basis for these dairy-adaptive traits. In addition, we will employ an array of assays and a bovine mammary epithelial cell infection model to examine the relevance of the novel biofilm phenotype to pathogenesis and resilience to antibiotic treatment. An understanding of the mechanisms involved may inform the development of novel therapeutic approaches targeting key adaptations required for survival. This hypothesis will be tested using a specific antibody approach and newly discovered effectors produced by other bacterial species in the dairy bovine microbiota. These data will result in the comprehensive characterisation of a novel dairy adaptive phenotype from its evolutionary origin to pathogenic function, and therapeutic potential.

Summary

Mastitis (infection of the mammary gland) is an important animal welfare issue and a major economic burden on the global dairy industry. Treatment with antibiotics is often ineffective due to bacterial biofilm formation, intra-cellular survival and antibiotic resistance. Alternatives to antibiotics are urgently needed but rational design of new therapeutic approaches requires improved mechanistic understanding of how bacteria cause disease. An array of bacterial pathogens can cause bovine mastitis including some that are adapted to survive and transmit in the dairy environment. For example, we have discovered that several major mastitis pathogens including Staphylococcus aureus, and Streptococcus agalactiae demonstrate enhanced growth associated with the ability to induce curdling in milk, and produce a milk-dependent biofilm phenotype. Of note, these phenotypes are not shared with human S. aureus or S. agalactiae isolates consistent with dairy niche adaptation. Here, using a combination of population level and molecular functional techniques, we will investigate key mechanisms supporting the ability of S. aureus to survive and proliferate within the dairy environment. In particular, we will dissect the basis for a novel milk-dependent biofilm phenotype, and investigate its relevance to pathogenesis and resilience to antibiotic treatment. The study will reveal key biological pathways and/or components required by bovine S. aureus to survive in the dairy environment which could represent novel targets for control of infection. The potential for therapeutic targeting of dairy adaptive traits for control of mastitis will be investigated using a specific antibody approach and novel anti-biofilm agents produced by other bacterial species that inhabit the dairy bovine microbiota.

Committee	Research Committee B (Plants, microbes, food & sustainability)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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