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Preventing dedifferentiation of neurons: a role for H3K9me- and HP1 associated heterochromatin?

Reference	BB/X00256X/1
Principal Investigator / Supervisor	Dr Tony Southall
Co-Investigators / Co-Supervisors	Dr Jelle van den Ameele
Institution	Imperial College London
Department	Life Sciences
Funding type	Research
Value (£)	912,173
Status	Current
Type	Research Grant
Start date	01/02/2023
End date	31/01/2026
Duration	36 months

Abstract

During development and tissue homeostasis, stem cells produce progeny that differentiate into specialised cell types. Epigenetic silencing of stem cell genes, or genes required for other cell fates, is important for these differentiated cells to maintain their specialised function and fate. Ageing and oncogenesis are accompanied by an erosion of these epigenetic marks, leading to aberrant gene expression and cell function. Substantial metabolic changes accompany these processes, but the exact relationship between metabolism, epigenetic erosion and cell fate changes or ageing remains incompletely understood. One key epigenetic mark involved in cell fate gene regulation is histone H3K9 methylation (H3K9me). Using Drosophila and mouse in vivo models, and taking advantage of innovative in vivo gene editing, chromatin profiling and metabolite sensing technology, we will investigate the role of H3K9me and HP1 heterochromatin in maintaining neural stem cell (NSC) gene repression in neurons, and investigate its relation to one-carbon metabolism, a central metabolic pathway involved in aging. First, we will comprehensively profile H3K9me and HP1 in neurons during development, dedifferentiation (using our in vivo neuronal dedifferentiation model) and ageing. Next, through multiplexed cell-type specific CRISPR-based epigenome engineering, H3K9me will be removed from a set of key NSC genes in neurons. We will explore whether the absence of H3K9me allows de-repression of the NSC genes and enhances or even causes dedifferentiation. In the last aim, we will characterise and genetically manipulate one-carbon metabolism enzymes to modulate levels of SAM and SAH, central metabolites involved in H3K9me, and assess the impact on H3K9me abundance and cell fate maintenance. Together, these studies will provide important insights into neuronal cell fate plasticity and mechanisms that prevent ageing in the brain.

Summary

As an organism develops, and when it repairs its tissues, stem cells divide to produce daughter cells that differentiate into specific cell types. These cells are the building blocks of the body and can have very diverse functions, such as hair follicle cells in the skin, or nerve cells (neurons) in the brain. As a cell differentiates, it shuts down expression of stem cell genes and turns on genes required for its specific function. It will also make sure that other genes (related to other functions or properties) are kept switched off. These 'epigenetic' mechanisms that prevent these genes from being expressed are very important. If silent genes are re-expressed, it can prevent the cell from functioning properly, cause cells to proliferate uncontrollably, or lead to cell death. It is becoming increasingly evident that erosion of epigenetic silencing, and the expression of unwanted genes, is a key driver of aging. Furthermore, metabolism, which is influenced by the genetic background, the environment and diet, can affect the epigenetic control of gene expression. Here, we will investigate the mechanisms that keeps neural stem cell (NSC) genes off in neurons. We have previously shown that the mutation of a specific transcription factor (a protein that binds to DNA to regulate gene expression) can cause developing neurons to have an identity crisis and revert back to a NSC-like state. This only happens in immature neurons, suggesting that there are epigenetic alterations that 'lock' NSC genes into a highly repressed state. A strong candidate is HP1 heterochromatin, which is characterised by the presence of the HP1 protein and a modification on histones (the proteins that DNA is wrapped around) called 'H3K9me'. Evidence from studies in Drosophila (fruit fly) and mouse cells show that HP1 heterochromatin is present at NSC genes in neurons. Our key hypothesis is that HP1 heterochromatin prevents neurons from 'dedifferentiating' back into NSCs. We will test this hypothesis and investigate how one-carbon metabolism, a central metabolic pathway involved in aging and histone modification, affects HP1 heterochromatin in neurons. We will first comprehensively profile HP1 heterochromatin in developing neurons, young adult neurons and old neurons. This will be done in Drosophila and mice using a technique called Targeted DamID that we developed. These data will determine whether mechanisms required for maintaining cell fate identity deteriorate with age. Then, to test our main hypothesis, we will use a cutting-edge technique called 'epigenome editing'. This utilises CRISPR-Cas9 technology (more widely known for its use in altering the sequence of DNA in the genome) that has been adapted to recruit effector proteins to specific sites in the genome. A histone demethylase will be recruited to NSC genes in neurons to remove the H3K9 methylation and prevent the formation of HP1 heterochromatin. We can then check whether NSC genes are de-repressed and whether it can cause or enhance dedifferentiation of neurons into NSCs. Finally, we will characterise and genetically manipulate one-carbon metabolism enzymes to modulate levels of SAM and SAH. These are the metabolites involved in H3K9me modification of histones and are known to change during ageing. We will assess the impact on H3K9me abundance and cell fate maintenance when the levels of these metabolites change in developing and ageing neurons. Together, these studies will provide important insights into neuronal cell fate plasticity and mechanisms that prevent ageing in the brain.

Committee	Research Committee C (Genes, development and STEM approaches to biology)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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