Award details

Towards on-column monitoring of protein purification by ATR-FTIR and Raman Spectroscopy

Reference	BB/R019533/1
Principal Investigator / Supervisor	Professor Bernadette Byrne
Co-Investigators / Co-Supervisors	Professor Sergei Kazarian
Institution	Imperial College London
Department	Life Sciences
Funding type	Research
Value (£)	199,240
Status	Completed
Type	Research Grant
Start date	01/12/2018
End date	31/05/2020
Duration	18 months

Abstract

unavailable

Summary

Biopharmaceuticals, proteins used as drugs, are an emerging area in the treatment of a range of diseases including rheumatoid arthritis and cancer. While these therapeutics have been shown to be very effective, they are also extremely expensive (~£10,000s/treatment), due to the complex, large scale production processes required. The most expensive step is Protein A affinity capture. Previous research by our group has utilised a specialized technique called Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy to explore the causes of Protein A resin decay. The research outlined in this proposal aims to further characterise binding capacity decay using this approach and also develop the approach to make this a tool that will be useful to scientists in an industrial bioprocessing environment. Our direct monitoring approach will provide much greater control into Protein A purification and has the potential to extend resin life time, saving production costs.

Committee	Not funded via Committee
Research Topics	Industrial Biotechnology, Structural Biology
Research Priority	X – Research Priority information not available
Research Initiative	Follow-On Fund Super (SuperFOF) [2012-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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