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Defining mechanisms of mucosal innate defence using the X. tropicalis tadpole

Reference	BB/M021688/1
Principal Investigator / Supervisor	Professor David Thornton
Co-Investigators / Co-Supervisors	Dr Eamon Dubaissi, Professor Richard Grencis, Professor Ian Roberts
Institution	The University of Manchester
Department	School of Biological Sciences
Funding type	Research
Value (£)	433,306
Status	Completed
Type	Research Grant
Start date	01/07/2015
End date	30/06/2018
Duration	36 months

Abstract

Studying innate immunity at mucosal surfaces in mammals is technically difficult because these surfaces are located internally, and adaptive immunity complicates the picture. Here, we will use the Xenopus tropicalis tadpole as a model system to explore the innate defence function of the mucus barrier that overlays the tadpole skin. Importantly, this model will allow us to study innate defence mechanisms in the absence of adaptive immunity, which does not develop until metamorphosis. We have shown that the tadpole skin secretes a mucin, Otogelin, which forms the molecular framework of a mucus barrier; similar to mucus that protects the mucosal surfaces in mammals. We have identified other mucus components (IgG Fc gamma binding protein (FCGBP), Intelectin and Wap) that have been implicated in mucosal innate defence. These molecules have homologues in mammalian mucus and yet their innate defence roles remain unclear. We will use the X. tropicalis model system to define roles for these molecules in the innate defence of the tadpole against infection and provide insight to their roles in other organisms, including man. We will first knock down the individual molecules and assess the effectiveness of the mucus barrier. We will challenge tadpoles with Aeromonas hydrophila, which we have shown causes infection of tadpoles at high dose. Depletion of these molecules should render tadpoles more sensitive to infection. We will investigate the structural properties of the mucus with and without these molecules. We will also establish their antimicrobial activity, in protecting the tadpole from infection and use recombinant proteins to test their potency. Mucus contains other proteins implicated in innate defence. We will use this system to identify and define their roles. Uncovering fundamental mechanisms of innate defence may lead to novel strategies and targets to treat disease, and discovery of novel antimicrobial proteins will be useful in the fight against infection.

Summary

Pathogens are disease causing microorganisms and viruses, which represent a severe challenge to the health and survival of animals. When animals succumb to infectious disease, it is because pathogens have breached the body's natural defence mechanisms. Broadly, these defences fall into two types, innate and adaptive immune defences. Adaptive immunity is a specific defence, where antibodies generated against a particular pathogen are used to rapidly respond to infection. However, it is advantageous for animals if pathogens never have the opportunity to infect the body in the first place. Innate immunity (non-specific immunity) is the first line of defence against infection and includes the generation of physical barriers to entry and the production of molecules that actively destroy pathogens. One such physical barrier used by exposed tissues in the body (e.g. lungs and gut) is the production of a mucus layer. Mucus traps pathogens preventing them from accessing the underlying cells and also contains other innate defence molecules such as antibacterial agents. It is important to study mucus to understand how it functions and what happens when it becomes dysfunctional, and is breached. Severe diseases can arise due to lack of a properly functioning mucus barrier; these include inflammatory bowel disease, stomach ulcers, and asthma. Mammalian models, including mice, are often used to study mucus in the lungs and gut. However, simple non-mammalian organisms can also provide valuable insight into evolutionarily conserved mechanisms of mucus function. In this proposal, we introduce the frog, Xenopus tropicalis, and specifically the mucus on the surface of the tadpole stage, as a model to study innate defence. X. tropicalis is a model organism commonly used in developmental biology to understand basic concepts in how embryos develop and the molecular processes that occur. In recent years, we have been studying how the embryonic skin changes as the embryo develops into a tadpole, prior to becoming a frog. We have identified a number of cell types in the tadpole skin, including two types of secretory cells that together secrete molecules that form a mucus layer over the surface of the skin. We are proposing that this simple model can be used to study aspects of mucosal innate defence that will be applicable to other organisms, including humans. Indeed, we have identified a number of innate defence molecules in the tadpole mucus that have known homologues in humans and yet their functions are largely unexplored. In this grant proposal, we intend to interrogate their functions through a number of means. The X. tropicalis tadpole model has some distinct advantages in that the skin directly faces the environment and so conditions can be altered to observe the effects. This is not the case with mammalian mucus barriers, which are usually found on tissues within the body and are thus more difficult to access. In addition, X. tropicalis tadpoles have yet to acquire adaptive immunity (which happens later in their development) so any observed effects will be due to innate defence mechanisms, rather than a more complicated, combined adaptive response to infection. We intend to genetically alter the expression of the innate defence molecules in the tadpoles skin mucus layer and then challenge the tadpoles with a potential pathogen found in their native environment (the bacterium, Aeromonas hydrophila), in order to understand the importance of each molecule. We will look at their structural roles in the physical barrier and their potential functional roles as anti-microbial agents. We aim to advance the understanding of how the tadpole defends itself against infection and ultimately how evolutionarily conserved innate defence mechanisms function in mucus barriers. This could potentially lead to new targets for treating disease.

Impact Summary

Studying the innate defence mechanisms of X. tropicalis tadpoles will have impact beyond its academic significance. Understanding how the tadpole defends itself against infection will uncover fundamental principles in the innate defence of this organism. A potential impact of our research concerns global amphibian decline. This is a phenomenon driven by a number of factors and chief among them is disease and the impact of global warming. Using an amphibian model such as X. tropicalis to study natural innate defence mechanisms may give new insight into how disease can be tackled and the effect of climate change on innate defences to infection. Amphibian conservation may benefit from our research through generating publicity and awareness of the problems that amphibian's face, whilst our own research may benefit from such a partnership. These studies will also generate new discoveries about the biology of mucosal surfaces that are relevant to animal health. We have identified a number of molecules secreted from the tadpole skin that we expect to be involved in innate defence and furthermore a number of these molecules, in humans, show altered expression or mutation in disease. Investigating their functional roles may result in new clinical targets for the development of therapeutics. This would ultimately benefit patients suffering from debilitating mucosal diseases such as inflammatory bowel disease, gastric ulcers and asthma, but may also benefit the pharmaceutical and biotechnology companies that will have new drug targets; better understanding barrier organisation may aid with improved drug delivery across mucosal surfaces. One of the molecules we have identified is potentially a novel antimicrobial protein and we will be testing its activity in this regard. If these activities were proven then there would be potential for commercial exploitation, which would benefit the University of Manchester in terms of its goal to develop impact from research and also economically. Also, there is a pressing societal need to develop new types of antimicrobial molecules to combat increasing antibiotic resistance, which is a global issue. Identifying natural antimicrobials is one strategy and our research could help in this regard. The findings of our research may also be important in terms of the development of the model as a platform to study disease and to screen novel therapeutics. The tadpole skin is a mucosal surface and contains motile multi-ciliated cells, so we envisage that it could be used to mimic both mucosal disease and diseases of cilia motility (ciliopathies). Such a system may be useful for screening new molecules for efficacy and therefore our research may benefit the pharmaceutical industry and clinicians interested in developing therapies for these diseases. The researcher Co-I, Eamon Dubaissi, has a keen interest in the translational impact of research and has attended many courses and workshops in this field. He intends to continue to develop his interest in this area, which may be of economic and societal benefit in future, either within this research area or elsewhere. During the project, we expect to have societal impact, particularly in the local area, with active participation in public engagement events. All the applicants have taken part in public engagement events in the recent past and are keen to do so in future. Likely beneficiaries include the local community and also young students who will have the opportunity to interact with scientists and gain an insight into a research career and why research is important for everyone in society. Public enthusiasm for, and understanding of, science, is of benefit to all.

Committee	Research Committee A (Animal disease, health and welfare)
Research Topics	Immunology, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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