Award details

PeriTune - a clonal optimisation platform

Reference	BB/M011259/1
Principal Investigator / Supervisor	Dr Neil Dixon
Co-Investigators / Co-Supervisors
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	121,512
Status	Completed
Type	Research Grant
Start date	01/04/2015
End date	31/03/2016
Duration	12 months

Abstract

The aim of this collaboratory Early Stage Feasibility project is to develop a protein expression optimisation platform for the robust production of biologics. This platform will enable key bottlenecks and manufacturability challenges of recombinant proteins used in Industrial Biotechnology to be addressed. The platform will utilize the tuneable gene expression control technology RiboTite, developed at the University of Manchester. This technology operates at the level of translation initiation and permits cellular-level tuneable control of gene expression. Clones developed through the optimisation platform will be validated, demonstrated at scale, and assessed for manufacturability.

Summary

A variety of micro-organisms including E. coli and S. cerevisiae (baker's yeast), are used in commercial bio-production processes to manufacture a number of products, ranging from high-value low-volume products, such bio-therapeutics (e.g. recombinant insulin), to mid-value products, (e.g. biocatalysts and specialised chemicals), to low-value bulk commodity products (e.g. succinic acid and biofuels). This project will seek to develop a robust clone optimsiation platform for the potential use and application in the above industrial sectors, and so would be of great benefit in a number of applied and fundamental areas of biological and biomedical R&D.

Impact Summary

As described in proposal submitted to TSB

Committee	Not funded via Committee
Research Topics	Industrial Biotechnology, Microbiology, Synthetic Biology
Research Priority	X – Research Priority information not available
Research Initiative	Industrial Biotechnology Catalyst (IBCAT) [2014-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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