Award details

Exploiting next generation sequencing technologies to understand pathogenicity and resistance in Fusarium oxysporum

Reference	BB/K020730/2
Principal Investigator / Supervisor	Dr Richard Harrison
Co-Investigators / Co-Supervisors
Institution	National Inst of Agricultural Botany
Department	Centre for Research
Funding type	Research
Value (£)	39,874
Status	Completed
Type	Research Grant
Start date	01/02/2016
End date	31/03/2017
Duration	14 months

Abstract

unavailable

Summary

unavailable

Impact Summary

Onions are grown in virtually every country in the world and have a production value of US$ 24,698M. However, considerable yield losses occur due because of Fusarium basal rot caused by F. oxysporum f.sp. (forma speciales) cepae (FOC), and in the UK this is equivalent to economic losses of approx £11M per annum. The major aim of this proposed research is to provide information, tools and resources which will lead to more effective and sustainable control of this disease. Primarily this will be through the development of genetic resources and markers to enable the breeding of FOC-resistant onions. However, we will also identify genetic markers to distinguish FOC from non-pathogenic F. oxysporum isolates or other f.spp. affecting different crops which will ultimately lead to tests for detection and quantification the pathogen. This integrated approach to basal rot control will therefore benefit a wide range of stakeholders and also addresses the BBSRC strategic priority area of sustainable crop production, particularly with respect to reducing chemical usage and waste. Commercial beneficiaries of the research will include plant breeders, seed producers and growers. The primary and immediate beneficiary will be Nickerson-Zwaan (NiZ) who is the industry partner in the project as they will have access to unique onion lines which they will utilise to develop elite FOC resistant cultivars. Their involvement and investment ensures that there is a direct route for delivery of the research through their existing global product development and marketing infrastructure. This process will be accelerated by the information, tools and resources generated in the project which include the identification of FOC resistance loci and associated genetic markers in onion, the production of new onion populations segregating for FOC resistance and pre-breeding onion lines. Although NiZ will have access to these resources first, these will also be made publicly available for the benefit ofother onion breeders. Onion mapping populations, genome data and availability of other genetic resources in particular should facilitate breeding for other traits such as resistance to other disease and pests and daylength response. Markers for pathogenicity in Fusarium will also be an immediate output and could be informative for identifying effector-based resistance in other crops affected by F. oxysporum which will again benefit breeding companies. Growers will benefit from the research as in due course they will have access to Fusarium resistant onion cultivars not previously available. This will give them a more sustainable and attractive option for basal rot management as chemical control of the disease is difficult, inefficient, becoming more undesirable and subject to loss of products through legislation. Hence there will be reduced losses and wastage due to bulb rotting in field or store with concomitant increased economic returns. The markers for Fusarium pathogenicity will also provide a platform for developing diagnostic and quantitative tests for the pathogen in soil, onion seed, sets and bulbs. This will also benefit growers and crop consultants as they will be able to determine the health of their planting material and select appropriate pathogen-free fields for onion growing. They will also be able to assess whether bulbs at harvest are FOC-free and hence make decisions about their storage capability. The research towards developing onion lines resistant to onion basal rot and detection tests for FOC in this proposed research therefore represents two different approaches in developing a more sustainable integrated strategy to reduce Fusarium basal rot losses. The public will benefit by having access to high quality onions grown with reduced pesticide inputs. The research will also result in the generation of a wealth of data associated with onion and Fusarium genetics and eill hence benefit researchers and academics working in these areas.

Committee	Not funded via Committee
Research Topics	Crop Science, Microbiology, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	Horticulture and Potato Initiative (HAPI) [2012-2014]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/K020730/1 Exploiting next generation sequencing technologies to understand pathogenicity and resistance in Fusarium oxysporum

BB/K020870/1 Exploiting next generation sequencing technologies to understand pathogenicity and resistance in Fusarium oxysporum

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