Award details

IMPROMALT: Improving winter malting barley quality and developing an understanding of the interactions of introgressions with genetic background

Reference	BB/K007025/1
Principal Investigator / Supervisor	Dr Keith Gardner
Co-Investigators / Co-Supervisors	Professor Ian Mackay
Institution	National Inst of Agricultural Botany
Department	Centre for Research
Funding type	Research
Value (£)	67,036
Status	Completed
Type	Research Grant
Start date	01/05/2013
End date	31/03/2019
Duration	71 months

Abstract

We will augment existing genotypic and phenotypic information upon elite UK barley varieties to refine key malting quality QTL to more tightly defined intervals. We will then utilise the resources of the UK barley breeding industry to conduct a targeted introgression programme to develop pre-competitive germplasm that combines the top malting attributes of spring barley varieties into winter varieties to improve the sustainability of UK malting barley in the threats of climate change and land competition through the earlier maturity and greater yield of the winter crop. We will utilise the genetic resources developed in this proposal to fine map the QTL and generate a candidate gene list that we will then explore through phenotypic, expression and sequence analyses to identify the likely causal polymorphism in the main candidate for a target. In the process, we will develop QTL near-isogenic pairs, the most tightly defined of which we will use in a time-course experiment to sample the transcriptome at daily stages following imbibition in the malting process.. We will combine RNA-Seq analysis with alignment against the genomic and transcript assemblies being developed for barley, taking account of information being developed under the recently funded barley genome sequencing project (BB/I00663X/1), to align regions of SNP diversity against the genome to identify the gene. The project will not only provide germplasm that will ultimately help sustain the production of a product that contributes over £2.4 billion annually to the exchequer and accounts for 23% of all UK exports in the Food and Drink sector but also provide knowledge and resources to derive a fuller understanding of the action and interaction of genes to provide good malting quality.

Summary

Maltsters, brewers and distillers are concerned about the long-term sustainability of the barley crop. Seasonal problems in many parts of Europe resulted in a restricted malting barley supply that has only just been alleviated by an above average harvest in Argentina. Within the UK, drought conditions resulted in reduced barley crop quality, i.e. higher protein samples, particularly in Eastern England, where much English malting barley is sourced. Under predicted climate change scenarios, such drought conditions are likely to become more frequent and will affect the spring crop much more than the winter crop, which can escape the worst effects of summer drought through a much earlier maturity. Whilst winter barley might therefore provide a more consistent supply, the proportion bought by English maltsters has declined by over 25% over the past 20 years. This decline is due to the reduced quality level of the winter crop compared to the spring so that distillers can produce 16 more litres of raw spirit per tonne of malt on average from the latter. For an industry predicted to use 600,000t of barley from the 2012 harvest, this is a highly significant difference in production efficiency. All current UK winter barley malting varieties have been derived from Maris Otter, first recommended in 1965. Maris Otter combined the spring malting quality attributes of an older variety, Proctor, with the winter habit of Pioneer. Proctor was the major spring malting variety in the UK for many years but the introduction of Triumph was a quantum leap forward for the spring crop in terms of both quality and yield. In a previous project, we have analysed DNA fingerprints of UK spring and winter barley malting cultivars to identify genetic differences between the two crops that are associated with malting quality. Whilst plant breeders have previously tried to introgress spring quality attributes into winter barley, they have relied on chance events to assemble the right genes, which isan impossible task when the crops differ at thousands of genes. But we now have the knowledge and tools to conduct the introgression of spring attributes into winter barley in a highly targeted manner to test the hypothesis that their introduction will improve winter malting quality. The germplasm emerging from this proposal will then be used by the plant breeding partners of the project in further rounds of crossing and selection to develop improved winter malting quality cultivars that approached the spring quality levels but in a suitable agronomic background for contemporary farming practise and would thus re-generate interest in using winter barley for malting for use in brewing and distilling. As indicated in the previous paragraph, greater use of the winter crop is likely to provide a more consistent supply of malting barley in the future. As malting supplies are becoming tighter due to a variety of market factors, a switch to the higher yielding winter crop would also mean that the effects of competition for land for more profitable crops would have a less pronounced effect upon malting barley supply. As six row barley varieties tend to have a higher yield than two row, a longer term aim is to develop six row malting types that would further decrease the land area required to secure a malting barley supply.

Impact Summary

The major beneficiaries of this research will be: The plant breeding community. Initially this will be restricted to the Consortium members, who will be able to access the germplasm and markers to gain a competitive edge in developing new varieties that will increase their royalty earning potential. This will spread to other plant breeders worldwide through either the use of the improved winter varieties in their own crossing programmes and/or the eventual public release of the markers and protocols for selecting improved varieties. The farming community. Increased usage on winter malting barley would extend the market opportunities for growers and enable them also to spread their harvest load with an earlier maturing crop. Under predicted climate change scenarios, the drought escape mechanism provided by the early maturity of winter barley means that the crop is less susceptible to large fluctuations in grain protein content so that farmers will be more likely to secure a planned-for malting premium. Maltsters, brewers and distillers. This end user sector will benefit as the development of improved winter barley cultivars means that it has a wider pool of material to select from and, within limits, can effectively switch from one crop type to the other to take account of seasonal variations differentially affecting the overall quality of one, such as the Eastern region barley crop of 2011. Currently, all winter barley malting varieties produce epiheterodendrin and are thus unsuitable for the distilling market. The introgression of the epiheterodendrin null character into the winter barley crop means that the crop can then be considered for use in the distilling industry, opening further market opportunities for Scottish farmers as well as improving the long term sustainability of the industry. Society. The distilling sector alone contributes over £2.5 billion to the exchequer annually and so contributes strongly to the overall benefit of UK society. In addition, the industry employs around 10,000 directly and 40-50,000 indirectly. The brewing industry contributes over £3 billion to the exchequer annually and accounts for approximately 600,000 direct and indirect employment so the sustainability of the malting, brewing and distilling sector contributes massively to the UK economy.

Committee	Research Committee B (Plants, microbes, food & sustainability)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	LINK: Responsive Mode [2010-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/K008188/1 IMPROMALT: Improving winter malting barley quality and developing an understanding of the interactions of introgressions with genetic background

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