Award details

Developing molecular biology kits for more sensitive diagnostics and gene expression analysis

Reference	BB/J021601/1
Principal Investigator / Supervisor	Professor Tamas Dalmay
Co-Investigators / Co-Supervisors	Professor James Gray
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	119,930
Status	Completed
Type	Research Grant
Start date	30/08/2012
End date	29/08/2013
Duration	12 months

Abstract

unavailable

Summary

Molecular biology kits are widely used for research and diagnostics and are a multibillion dollar industry. Many of these kits use enzymes that modify DNA and RNA. Improving the accuracy and sensitivity of these enzymes can provide a kit with the unique selling point. In BBSRC-funded research, we have developed a novel approach to study DNA or RNA modifying enzymes using Next Generation Sequencing (NGS). This rapid, low-cost method was used to identify factors that improve the activity of DNA polymerases used in PCR, and RNA ligases used in kits that capture small RNAs (sRNAs). We plan to develop a High-Definition (HD) method that more accurately captures RNA molecules, which have been linked with cancers and disease. Funding is also requested to develop a PCRmax technology to generate diagnostic kits with increased sensitivity. PCRmax technology is a rare opportunity to develop a method leading to a step change in PCR efficiency.

Committee	Not funded via Committee
Research Topics	Technology and Methods Development
Research Priority	X – Research Priority information not available
Research Initiative	Follow-On Fund (FOF) [2004-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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