Award details

Mapping resistance to Campylobacter in the chicken

Reference	BB/J006815/1
Principal Investigator / Supervisor	Professor Mark Stevens
Co-Investigators / Co-Supervisors	Dr Paul Hocking, Professor Peter Kaiser
Institution	University of Edinburgh
Department	The Roslin Institute
Funding type	Research
Value (£)	462,693
Status	Completed
Type	Research Grant
Start date	01/08/2012
End date	30/11/2015
Duration	40 months

Abstract

Campylobacter has long been considered to be a commensal in the chicken, and therefore at first consideration it would seem unlikely that there could be resistance to colonisation. However, we believe that rather than being a commensal, Campylobacter is actually an accomplished pathogen. It can induce typical innate immune responses (albeit that these are time- and magnitude-limited), it can be partially controlled by vaccine-induced adaptive immunity, and genetic resistance to colonisation exists in chickens. We have identified four Campylobacter resistance QTL in inbred lines 61 and N. Our overarching hypothesis is that QTL controlling resistance to Campylobacter are also circulating in commercial chickens, and that some of these will be in common with those identified in the inbred lines. For example, of the 4 QTL we identified for resistance to colonisation with Salmonella, two are circulating in commercial chickens. We will refine the Campylobacter resistance QTL in the inbred lines, identify candidate genes and nucleotide changes and analyse the functional consequences of this sequence variation. We will also perform whole genome association/genomic selection (WGA/GS) for Campylobacter resistance in commercial lines and then independently validate the WGA/GS results. This dual approach is required as we cannot apply a simple candidate SNP polymorphism approach in the commercial lines, based on the inbred line data, as we have no evidence that there is the same underlying linkage disequilibrium (LD) between the causal mutation and the SNP in the commercial lines. The overall aim is to identify markers (SNPs), candidate genes and ultimately causative mutations for resistance/susceptibility to colonisation with Campylobacter in chickens. The resistance-associated genotypes will inform commercial breeding programmes to reduce the incidence of Campylobacter contamination of poultry carcasses and thereby levels of this food-borne zoonosis in humans.

Summary

Food security is an increasing priority for the UK Government and food safety is a key component of this. Campylobacter is the most common cause of food poisoning in the UK and is responsible for an estimated 321,000 estimated cases in England and Wales in 2008, with over 15,000 hospitalisations and 76 deaths. Campylobacter accounts for a third of the cost of food-borne illness in England and Wales, estimated at £583 million in 2008. It is found mainly in poultry and control of the organism in this reservoir is expected to lower the inclidence of human infection. "Campylobacter is a highly complex organism and we will only be able to understand it more fully and to overcome the threat it poses by deploying world-class research across microbiology, immunology and molecular biology... Addressing the Campylobacter challenge involves two of the three BBSRC strategic priorities of food security and basic bioscience underpinning health, in addition to exploiting systems approaches and new research tools." Professor Douglas Kell, BBSRC CEO. The UK Research and Innovation Strategy for Campylobacter, 2010-2015, is joint funded by the FSA, BBSRC, DEFRA, DARDNI and the Scottish Government. The overarching aim for the funders is to reduce the incidence of Campylobacter infections in humans, through reduction of the level of the bacterium in its farm animal hosts and the potential for cross-contamination in the food chain. Two of the main research priorities are understanding colonisation in the chicken and the chicken's immune response, and development of greater resistance to Campylobacter colonisation in the chicken. However, research in either of these areas was not included in a recent joint call for research into control of Campylobacter. The availability of chickens with significant innate resistance to Campylobacter colonisation would represent a safe, cost-effective solution. We have shown that genetic variation influences resistance to Campylobacter colonisation inchickens, with one line able to reduce levels of the bacterium in the gut by up 10,000-fold relative to another line. Complex genetic analysis in these inbred chicken lines has indicated that some of the regions, or loci, associated with resistance overlie known bacterial resistance genes. In this proposal we will investigate these loci further to locate the beneficial genes or mutations. We will carry out further work to map resistance genes, coupled with using the latest methods that allow selection of genes throughout the whole genome. Using tools such as new generations of high density panels to detect single nucleotide polymorphisms (changes in a single base of DNA between two different individuals at the same site) (~750k can be analysed at once), we can identify animals with candidate resistance genes that can be used to select future generations of chickens with robust resistance and greatly reduced levels of Campylobacter colonisation. As recommended by the Strategy, we will carry out this genetic research in collaboration with industry (Aviagen, a UK-based company that produces the pedigree chickens from which are generated over 50% of the world's 50 billion chickens every year), since gene association studies must be implemented in poultry breeding programmes. It is likely that resistance to Campylobacter depends on multiple genes and the selection of resistant lines will depend on the identification of specific traits and candidate animals. Though potential also exists to control Campylobacter on farm via improvements to hygiene and biosecurity, dietary modification, novel antimicrobial agents and vaccination, we believe that the proposed project would allow a targeted and rapid approach to breeding lines of poultry more resistant to Campylobacter colonisation.

Impact Summary

The UK poultry industry faces numerous challenges in order to remain sustainable. These include the imminent move to more extensive rearing systems; the withdrawal of prophylactic and many therapeutic antibiotics, and other drugs such as anti-coccidials; resistance and residue problems with anti-helminthics. These challenges will all have an impact on poultry health, but also have the potential to impact on human health. For example, with an increased incidence of zoonotic pathogens in chickens, there is the potential to lead to an increase in these diseases in humans. It is important that poultry breeders are able to select for genetic improvement in performance when birds are reared in such environments. New and effective disease control requires detailed understanding of host-pathogen interactions. We have recently shown that, far from being a commensal of the chicken, Campylobacter is actually a very accomplished pathogen. Campylobacter can induce typical innate immune responses (albeit that these are time- and magnitude-limited), it can be partially controlled by vaccine-induced adaptive immunity in chickens, and genetic resistance to Campylobacter colonisation exists (we recently identified four resistance QTL in inbred lines). However, we still need to identify the genes and mutations that are responsible for the resistance phenotype and if these, or others, segregate in modern commercial pedigree chicken lines, as these lines form the basis of selection for the broiler birds we eat. The proposal is supported by Aviagen Ltd, the UK-based world's premier poultry breeding company that produces the pedigree chickens from which are generated over 50% of the world's 50 billion chickens every year. Aviagen is committed to improving the health status of their commercial broiler stock through selection for resistance to disease challenges, pathogens associated with food-safety and general fitness. Improving our understanding of the genetic basis of resistance to Campylobacter will complement current research in this area. Their motivation for supporting this research is to better understand these important food security pathogens with the ultimate objective of informing their ongoing breeding programme. If successful, the results from this project will allow a targeted and rapid approach to breeding lines of poultry more resistant to Campylobacter colonisation and infection. Beneficiaries from this research therefore include academics interested in the genetics of disease resistance, host-pathogen interactions and disease control. The support of Aviagen will ensure this transfer of knowledge from the academic environment to industry. Results will be published, when appropriate, in peer-reviewed scientific journals and will be presented at various conferences (e.g. the XIIIth Avian Immunology Research Group meeting, Edinburgh 2012 and the 7th International Chick Meeting in Japan, 2013). Effective uptake of the outcomes of our research are also facilitated by close links with the Biosciences KTN, who identified disease resistance and animal health as the top priorities for UK breeding industries. This work has the potential to impact on the sustainability of the poultry industry in the UK, and also to inform FSA and DEFRA policy. It also therefore falls within the remit of the BBSRC Sustainable Agriculture Strategy Board and priority for research on Global Food Security. Most importantly, this proposal falls squarely into the research priorities of the UK Research and Innovation Strategy for Campylobacter, 2010-2015, jointly funded by the FSA, BBSRC, DEFRA, DARDNI and the Scottish Government, the remit of the BBSRC 'Animal Disease, Health and Welfare' Committee and matches the BBSRC scientific priority of 'Animal Health'. Policy priorities of 'economic and social impact' and 'welfare of managed animals' will also be addressed by this research.

Committee	Research Committee A (Animal disease, health and welfare)
Research Topics	Animal Health, Animal Welfare, Microbial Food Safety, Microbiology
Research Priority	Animal Health, Global Security
Research Initiative	LINK: Responsive Mode [2010-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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