Award details

High Throughput Arrays of G Quadruplex DNA

Reference	BB/I015477/1
Principal Investigator / Supervisor	Professor Sir Shankar Balasubramanian
Co-Investigators / Co-Supervisors	Dr Geoffrey Smith
Institution	University of Cambridge
Department	Chemistry
Funding type	Skills
Value (£)	91,932
Status	Completed
Type	Training Grants
Start date	01/08/2012
End date	31/07/2016
Duration	48 months

Abstract

unavailable

Summary

A high density array comprising approximately one billion short nucleic acid sequences (aptamers) will be generated on a next generation sequencing system and tested as an assay platform to identify binding agents to a range of antigens. The initial focus of the project will study variations to a well-characterized G quadruplex sequence that is known to bind specific protein targets, and will explore the capacity of this sequence motif as a generalized scaffold for binding a range of protein and small antigens. The project will generate an alternative technology to SELEX for the display and identification of aptamers as a discovery tool for the characterization of specific DNA -small molecule and DNA-protein interactions, and will lead to the design of molecular probes that can be exploited to study biological hypotheses that are the subject of current interest.

Committee	Not funded via Committee
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	Training Grant - Industrial Case

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