Award details

A synthetic & recombinant approach to the production and characterisation of IAPV an associated agent of honey bee Colony Collapse Disorder

Reference	BB/G02040X/1
Principal Investigator / Supervisor	Professor Ian Jones
Co-Investigators / Co-Supervisors
Institution	University of Reading
Department	Sch of Biological Sciences
Funding type	Research
Value (£)	320,689
Status	Completed
Type	Research Grant
Start date	01/12/2009
End date	30/03/2013
Duration	40 months

Abstract

Infection by Israeli Acute Paralysis Virus (IAPV) has been suggested from recent metagenomic data of infected and non infected hives to be a major factor in honey bee Colony Collapse Disorder. The virus was only characterised in 2007 and has yet to be propagated in pure culture. As a result, study of its replication, assembly and pathogenesis, all of which would benefit an understanding of its role in CCD, is lacking. In the absence of a culture system fro IAPV, an alternate route to the production of virus particles is to use synthetic biology, the synthesis of the genome de novo based on available database sequences, followed by expression of the encoded open reading frames. Virus assembly will then occur as a consequence of expression. Such an approach has recently proved feasible for a related virus, RhPV, where the derived virus was also infectious allowing the study of relationship between sequence and pathogenicity. We have direct experience of this technology through a current award which uses it for the generation of empty FMDV particles. Our work has required novel sequence constructions which now give the highest levels of FMDV particle ever recorded for a recombinant system. We now propose to apply the same technology to the production of IAPV particles, to characterise them biochemically and to use them to generate the reagents necessary for a hive side diagnostic test for IAPV presence. The latter studies will be done in collaboration with the National Bee Unit in York who have particular expertise in assay development and their application in field conditions. In addition to providing a research base on IAPV and diagnostics our studies will also act as proof-of-principle for the application of similar technology to other bee viruses in the order Picornavirales.

Summary

Colony Collapse Disorder (CCD) is the name given to a catastrophic loss of honey bee colonies that has spread across the United States during the last 40 years and across Europe in the last 15 years. The loss of honey bees (Apis mellifera) through CCD has recently been attributed to infection by Israel acute paralysis virus (IAPV), a dicistrovirus whose spread or pathogenicity may be exacerbated by bee infestation with the Varroa mite. A focus on IAPV and the possibilities for its control is now appropriate but the virus has never been isolated in pure culture so genetic manipulation and production of the pure virus preparations is impossible, slowing progress in diagnostics and treatments. Recently it has been shown that the incorporation of the related Rhopalosiphum padi (wheat/oat aphid) dicistrovirus genome into the genome of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) leads to the synthesis of dicistrovirus virus particles. Essentially the dicistrovirus is being carried along by a larger virus so bypassing the need to establish its own infection. The use of baculoviruses to express single recombinant proteins such as viral proteins is well established and if the virus protein expressed is part of the structure of the virus they often assemble into virus particles. This is the case for positive strand RNA viruses, such as picornaviruses and dicistroviruses, where the entire genome translates to a single or twin polyprotein which then processes itself and assembles into virus particles. The system allows the synthesis of authentic dicistrovirus virus particles in insect ells without the need to establish a productive tissue culture system and provides a method for virus study that is independent of the ability to isolate the virus from the wild. Here we propose to use the recombinant baculovirus technology to produce Israel acute paralysis virus, the virus associated with CCD. We will use the virus particles produced to generate a panel of monoclonal antibodies and sue them to develop an easy-to-use hive-side test for IAPV infection. In addition we will investigate the relationship between virus strain and pathogenicity and define the translational products of the virus, essential for a full understanding of its action. The role of particular viral proteins in pathogenicity will also be studied through site directed mutagenesis coupled to quantitative infection assays. The work described will be done by the PI in collaboration with the National Bee Unit at the Central Science Laboratory, York who will provide expertise in virus measurement and the development of diagnostics to match the virology and recombinant protein expression expertise at Reading. Our work will thoroughly characterise this newly described bee virus and benefit the long term control of CCD.

Committee	Closed Committee - Agri-food (AF)
Research Topics	Animal Health, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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