Award details

An amphipathic reagent to extract stabilize and purify proteins

Reference	BB/G010412/1
Principal Investigator / Supervisor	Professor Timothy Dafforn
Co-Investigators / Co-Supervisors	Professor Roslyn Bill, Professor Michael Overduin, Professor Owen Thomas
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	414,728
Status	Completed
Type	Research Grant
Start date	01/05/2009
End date	30/04/2012
Duration	36 months

Abstract

Biopharmaceutical products represent the largest growth area in the pharmaceutical sector. These products offer a high degree of efficacy and selectivity compared to many conventional small molecule drugs making their development a high priority. However, although biopharmaceutics offer many health benefits, their production is a significant technical challenge. This is the result of a number of factors, but perhaps two of the most important being the difficult of specifically releasing the product from the feedstock and the low stability of the product. This project aims to continue our pioneering development of a reagent that addresses both of these issues. The reagent, a substituted polystyrene (SPS) is a highly amphipathic entity that has a number of remarkable properties that make it an exceptionally suited to downstream processing of biopharmaceutics. The reagent disrupts lipid bilayers to form a nano-disciodal structure in which the lipid bilayer is stabilised by a 'bracelet' of the SPS. The lipid-SPS assembly can also include membrane associated proteins allowing them to be stabilised in an entity that can be used in conventional chromatographic separations. The SPS can be easily removed from solution by a change is solution conditions leaving just the product solubilised in native membrane. In this project we will also demonstrate that SPS is multifunctional, being used to release products from the expression system and then acting as a stabilising agent for unstable products. The SPS has also been cleared by the FDA as a highly effective formulation and delivery system, allowing the potential to use a single reagent from cell disruption to administration. It therefore seems clear that, perhaps uniquely, SPSs can both improve product yield and purity of biopharmacuetic product as well as simplifying, and hence reducing the cost of the downstream process. This project aims to develop SPSs into a potent new entity for use in bioprocessing.

Summary

The development of new therapeutic agents has undergone a revolution during the last two decades. For 80 years medicines have almost exclusively consisted of relatively small chemicals. However, biotherapeutics agents have recently emerged as the fastest growing type of new drugs being developed. They include antibodies that are part of our body's immune systems that fight off infections. Antibodies are a type of molecule called a protein; these proteins are highly complex and very fragile in contrast to the chemicals that were used in medicines in the past. Most biotherapeutic agents in clinical use are drugs manufactured in microorganisms or substances that are produced by living organisms. They include antibodies and enzymes. Most biotech pharmaceuticals are recombinant proteins produced by genetic engineering. Specific examples include cytokines like insulin and interferons, recombinant enzymes that combat cystic fibrosis (dornase alfa) and heart disease (Alteplase), hormones (Erythropoietin and human growth hormone), clotting factors, vaccines and monoclonal antibodies. Biotech pharmaceuticals have major limitations. Many are difficult to purify intact, and are unstable with limited shelf lives. Also, some are not absorbable in a medically useful form through the gastrointestinal tract, lungs or skin. Impurity is also a common problem, as they most be purified from complex biological sources. Protein impurities can cause allergic reactions or alter therapeutic effects. Hence, production of a stable protein particle that can easily be purified is very important. We have developed a new solution that increases both the yield and stability of the biotherapeutic. The chemical (called SPS) that provides this solution can act in two ways that helps the production of certain biotherapeutcics. Firstly it can be used to help release the product from the microorganisms in which it has been made. Once released the SPS can act like a stabilising bracelet, wrapping around proteins that are not generally stable in water preventing them from loosing activity. Both of these abilities will greatly increase the efficiency of biotherapeutic production which should result in cheaper and more available drugs. As an added advantage the reagent is safe for use in human beings and has been shown to facilitate the uptake of drugs into the body, increasing their effectiveness. This again has the potential to reduce drug costs. This project aims to demonstrate the effectiveness of SPS in biotherapeutic development while at the same time develop protocols so that it can be easily adopted by companies making biotherapeutics.

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	Industrial Biotechnology, Pharmaceuticals
Research Priority	X – Research Priority information not available
Research Initiative	Bioprocessing Research Industry Club (BRIC) [2006-2012]
Funding Scheme	X – not Funded via a specific Funding Scheme

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