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SI-mediated phospho-regulation of pollen soluble inorganic pyrophosphatases

Reference	BB/G003149/1
Principal Investigator / Supervisor	Professor Vernonica E. (Noni) Franklin-Tong
Co-Investigators / Co-Supervisors	Dr Scott Andrew White
Institution	University of Birmingham
Department	Sch of Biosciences
Funding type	Research
Value (£)	444,506
Status	Completed
Type	Research Grant
Start date	16/02/2009
End date	15/05/2013
Duration	51 months

Abstract

This project has 3 specific aims to understand SI-mediated sPPase phosphorylation using an integrated programme of work. Aim (A) is to establish the sites on which Pr-p26.1a/b are phosphorylated using FT-ICR mass spectrometry and using site-directed mutagenesis. Phospho-mutant recombinant proteins will be tested using functional assays assessing how mutating phosphorylation sites affects ability to be phosphorylated, sPPase activity, and pollen tube growth, using comparisons with wild-type. Aim (B) is to clone, validate and analyse the poppy pollen CDPK(s) responsible for the SI-mediated phosphorylation of Pr-p26.1a/b. We will also carry out functional analysis of recombinant CDPKs to establish their ability to phosphorylate Pr-p26.1a/b, their relative efficacy in inhibiting sPPase activity, using Fiske-Subbarow sPPase assays and their effect on pollen tube growth regulation using site-directed mutagenesis and transient expression in pollen. Aim (C) is to determine the changes in the PPase reaction kinetics caused by phosphorylation. Steady-state kinetics, kinetic and binding studies will establish how PPase activity is inhibited by phosphorylation and whether Pr-p26.1a/b display different kinetic changes. Isothermal titration calorimetry will establish affinity of ligands that affect activity.

Summary

Self-incompatibility (SI) is an important mechanism in flowering plants, utilizing cell-cell recognition, to prevent self-fertilization, which would otherwise result in undesirable inbreeding. An understanding of processes involved could help plant breeders develop F1 hybrids more efficiently. In Papaver (poppy), the female part of the flower (pistil) secretes a protein which acts as a signalling 'ligand'. This interacts specifically with a self (S-) pollen receptor, allowing pollen to distinguish between incompatible and compatible partners. An incompatible (self) interaction triggers signalling in incompatible pollen, involving increases in intracellular calcium that acts as a 'second messenger' to tell incompatible pollen that it is been recognised as 'self'. This activates events resulting in inhibition of growth and cell death to ensure that self fertilization is not achieved. Inorganic pyrophosphatases (PPases) are ubiquitous enzymes that hydrolyse inorganic pyrophosphate (PPi) generated during many cellular processes. This provides a thermodynamic driving force for biopolymer synthesis (such as cell walls, DNA synthesis) and so is essential for all cell growth. Two poppy pollen sPPases (Pr-p26.1a/b) were recently shown to be phosphorylated during incompatible SI interactions and to play a role in regulating pollen tube growth. This was the first demonstration that eukaryotic sPPases can be phosphorylated and that their activity is inhibited by phosphorylation. Modification of proteins by phosphorylation comprises adding of phosphate molecules, and is a key mechanism to alter enzyme activity, allowing cells to respond to extracellular stimuli and to regulate many biological processes. Despite the essential nature of sPPases, virtually nothing is known about their regulation by phosphorylation, so this is an exciting new area. The proposed studies will provide important insights into this newly discovered area, which could have widespread significance with respect to control of how cells regulate their metabolism. The proposed project will investigate SI-mediated Pr-p26.1a/b phosphorylation, including identifying which amino acid sites they are phosphorylated on, identifying the enzyme(s) responsible for phosphorylation (known as kinases), involved, and the consequences of phosphorylation for sPPase function. This will provide an understanding of how the modification of these proteins by phosphorylation can regulate their activity. Biochemical assays will assess the impact of phosphorylation on sPPase activity to establish the important question of how phosphorylation inhibits sPPase activity. Plants have a unique family of kinases (calcium dependent protein kinases: CDPKs); they are implicated in being responsible for phosphorylating the poppy pollen sPPases. Identification of the CDPK(s) mediating sPPase phosphorylation will provide an important step forward in our understanding of these events. Investigating the SI-regulated sPPases using these approaches will provide important advances in understanding self-incompatibility mechanisms and the newly discovered modification of sPPase activity through phosphorylation.

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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