Award details

Systems analysis of the early phase of yeast bud formation using a combined experimental and theoretical approach

Reference	BB/G001855/1
Principal Investigator / Supervisor	Professor Andrew Goryachev
Co-Investigators / Co-Supervisors
Institution	University of Edinburgh
Department	Sch of Biological Sciences
Funding type	Research
Value (£)	278,315
Status	Completed
Type	Research Grant
Start date	01/02/2009
End date	30/11/2011
Duration	34 months

Abstract

The establishment of cell polarity is a fascinating phenomenon by means of which cells break their functional symmetry. Cellular polarization is a prerequisite for cell motility, division and differentiation into mature functional forms, such as neurons, phagocytes and polarized epithelial cells. The loss of polarity is one of the first signs of cancerous transformation. Therefore, the understanding of how initially symmetric cells acquire polarity is of significant importance for both fundamental cell biology and practical biomedical applications, such as cancer research. Formation of yeast bud is one of the best experimentally studied scenarios of the cell polarity establishment in eukaryotic cells. The early symmetry-breaking stage of this process is the emergence of a cluster of activated small Rho GTPase Cdc42 that marks the presumptive bud site on the membrane. Recently we investigated the reaction dynamics of Rho GTPases in dense membrane-bound protein clusters, such as the incipient yeast bud, with specific focus on the control of GTPase nucleotide cycling by their regulatory molecules, GEFs and GAPs. Building on this advance, we will now develop a cell-scale computational model that biophysically describes the emergence of the Cdc42 cluster in the early phase of the yeast bud formation. We will comprehensively interrogate the model to address a number of important biological questions: Which molecular interactions drive accumulation of the activated Cdc42 on the membrane? Which molecular mechanisms define the spatial localization and temporary dynamics of the cluster? What determines the uniqueness of the bud throughout the cell cycle? Our theoretical predictions will serve as the guidelines for our experimental collaborators whose data will be fed back into the model development. By iterating this process, we will arrive at a deeper understanding of the cell polarity establishment in yeast and eukaryotes in general.

Summary

The ability of biological cells to actively respond to their environment is one of the most fundamental properties of the living matter. A class of such responses, termed polarization, results in the formation of a detectable 'head-to-tail' axis within the cell. For example, a pulse of growth-stimulating chemicals may cause an initially symmetric cell to undergo a morphological transformation by means of which it acquires a flat and wide front end and a trailing narrow back end. Once polarized in such a way, the cell can persistently migrate towards the source of the inducing chemical. The cellular polarity status is intimately related to the health of the cell. Loss of the normal epithelial polarity of cells that form the lining of internal organs, such as intestine, ovaries or kidneys, will inevitably cause cellular proliferation. Such an overgrowth may become a malignant tumor. If a normally non-polar cancer cell manages to acquire the migratory-type polarity, it becomes motile and may cause the spread of cancer through metastases. The understanding of the mechanisms that underlie the polarity establishment is therefore highly important for the biology in general and the health research in particular. The major question of cell polarity that still baffles experimental and theoretical biologists is: What is the nature of the cellular compass? This 'device' is apparently located on the cellular membrane where it can perceive the external directional cues and then signal to the cellular insides. The latter is achieved by physically marking a membrane domain that is destined to become 'front' or 'back' with the specific protein complexes. The details may vary from one cell type to another, but the principle of using self-assembling clusters of protein complexes to differentiate specific areas from the rest of the cell membrane appears to be universal. Striving to understand these complex processes, my group uses mathematical and computational modeling as researchtools. To quantitatively characterize the underlying molecular mechanisms, we recently developed a model that describes the local chemical kinetics within the protein complexes that form these clusters. Our model shed light on the biochemical machinery that underpins the fast assembly and disassembly of such complexes. To explain how the entire clusters emerge in response to the extracellular stimuli, we have built a cell-scale model that together with reaction dynamics also incorporates the transport of molecules on the cell membrane and between the membrane and the cytoplasm. This is a considerably more complex endeavor and the careful choice of a specific system is crucial for its success. Based on the availability of experimental data as the major criterion, I selected the formation of baking yeast bud. Individual molecules and interactions that contribute to the emergence of yeast bud had been carefully described in the literature but the overall understanding of this complex developmental process is still lacking. My systems modeling will bridge this gap in our knowledge by bringing individual elements together to form the complete picture. Our preliminary results indicate that a nonlinear process known in chemistry as the autocatalysis is responsible for the creation of the protein cluster that will eventually develop into the fully grown yeast bud. More work, both experimental and theoretical, is necessary before our model can generate concrete experimentally testable predictions. This work will be done in a close collaboration with the internationally renowned yeast biologists, Profs. Erfei Bi of the University of Pennsylvania and Daniel Lew of Duke University. Their experimental results will be used by us to further improve the model while our predictions will inform their experiments. This project will serve as an example of a systems biology approach to complex biological problems to be followed by other biomedical researchers.

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	Microbiology, Systems Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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