Award details

Microarray-based discovery of plant growth-regulatory genes

Reference	BB/F020759/1
Principal Investigator / Supervisor	Professor Nicholas Harberd
Co-Investigators / Co-Supervisors	Professor Richard Mott, Professor Ioannis Ragoussis
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	379,505
Status	Completed
Type	Research Grant
Start date	01/10/2008
End date	30/11/2010
Duration	26 months

Abstract

This proposal describes a route to the identification of previously unknown plant growth-regulatory genes, and the proteins that those genes encode. Previous analyses have identified the DELLA proteins (DELLAs) as playing a major role in integrating the control of plant growth in response to diverse regulatory signals. However, it is also clear that additional unknown proteins play important roles in this process, and likely that these proteins are genetically uncharacterised (because loss-of-function mutant phenotypes are undetectable in the presence of functional DELLA proteins).This proposal therefore exploits a recently developed Arabidopsis mutant line that lacks all five of the Arabidopsis DELLAs (GAI, RGA, RGL1, RGL2 and RGL3). Large fast-neutron irradiation-mutagenised M2 populations of this line have been generated, and will be screened for novel growth mutations (conferring elongated hypocotyl (hy), cotyledon expansion, ABA-sensitivity and salt-resistance phenotypes). Fast-neutron mutagenesis preferentially generates deletion mutations of 0.8-12.0 kb in size, and hybridisation of genomic DNA to whole-genome 'tiling' microarray chips and subsequent bioinfomatic data analysis permits detection of such deletions (see Case for Support). We will optimise these methods for the rapid and reliable identification of the candidate genes mutated in the novel growth-regulatory mutants described above, and subsequently confirm gene identification via PCR-based comparison of independent alleles and/or transgenic complementation. The resultant identification of novel growth-regulatory genes and the proteins that they encode will be an important step towards the overall goal of a systematic understanding of the mechanisms via which the growth of plants is regulated.

Summary

Plants are the ultimate source of all human food and (as fossil fuels) the majority source of the energy upon which modern civilisation depends. It is therefore of crucial importance that we fully understand how the growth of plants is controlled. Previous work has identified a family of proteins (known as the DELLA proteins) that restrain the growth of plants in response to signals from the environment. These signals warn plants of environmental conditions that are not optimal for growth. Plants then use their DELLA proteins to adapt their growth accordingly. However, it is clear that additional proteins control plant growth, and that the identification of these additional proteins has previously been hindered because their effects are masked by the effects of the DELLA proteins. This proposal firstly describes how genetics can be used to unmask the effects of these previously unknown growth-regulatory proteins, and secondly describes how harnessing the latest advances in plant genome biology and computational analysis will enable us to identify the genes encoding those growth-regulatory proteins. The systems that regulate the growth and development of organisms are often physiologically 'buffered'. Consider an imaginery case where growth is regulated by two distinct mechanisms, A and B. It is frequently found that a gene mutation affecting mechanism B only has a detectable effect on growth when mechanism A is not functioning. Thus, when A is functioning, B is genetically 'invisible' because mutations in genes involved with B have no effect on growth (no detectable 'phenotype'). The way to circumvent these problems is to perform mutant screens in genetic backgrounds lacking A. Accordingly, we are proposing to identify novel growth mutants from a genetic background in which the DELLA growth-regulatory mechanism (mechanism A in our imaginery case) is missing. The new mutants will be generated using fast-neutron mutagenesis. Fast neutrons are particularly useful mutagens because they tend to generate small deletions of contiguous segments of DNA from plant chromosomes. These deletions are usually small enough to be be contained within (or encompass) single genes. It has recently become possible to detect such deletions at the molecular level. Hybridization of DNA from deletion mutants to 'chip-array' representations of the entire plant genome (and subsequent computational analysis of the resulting data) allows precise detection of the location of the deletion mutation, and hence identification of the gene affected. However, whilst these methods work (see Case for Support), they are very new and still relatively crude. We therefore propose further methods improvement, both of the hybridisation reaction and of the software that does the data-analysis, so as to make the detetion of mutated genes both reliable and routine. The proposal therefore outlines the development of a mutation detection 'pipeline' that quickly leads from initial identification of novel mutations affecting growth, through microarray-based detection of the molecular site of those mutations, to the eventual identification of previously unknown growth-regulatory genes (and protein gene products). Once these previously unknown growth-regulatory proteins and genes are identified we will have moved a step further towards understanding how plant growth is controlled. We already know that growth is controlled in response to a complex network of different signals from both the environment and from the plants interior. Somehow, this network of signalling inputs is integrated into a single growth output. How this crucial integration is achieved is currently only incompletely understood. The work described in this proposal will enable the identification of proteins (additional to the DELLA proteins) that perform this integrating role, and will thus contribute to the ultimate goal of a full understanding of how plant growth control is achieved.

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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