Award details

SysMO Sulfolobus (Schleper)-WesterhoffManchester

Reference	BB/F003536/1
Principal Investigator / Supervisor	Professor Hans Westerhoff
Co-Investigators / Co-Supervisors	Dr Frans Bruggeman, Professor Jacob Snoep
Institution	The University of Manchester
Department	Chem Eng and Analytical Science
Funding type	Research
Value (£)	151,201
Status	Completed
Type	Research Grant
Start date	14/11/2007
End date	13/11/2010
Duration	36 months

Abstract

Even slight differences between the rates of individual reactions in major metabolic pathways should cause rapid accumulation or depletion of intermediates with potentially deleterious effects. With a change in temperature, the rates of individual reactions in metabolic pathways must therefore change by precisely the same extent. Organisms could adapt by (i) having identical temperature coefficients of the enzymes, (ii) metabolic regulation, (iii) adjusting Vmax's (e.g. through enzyme phosphorylation), (iv) adjusting translation or protein stability, (v) adjusting transcription or mRNA stability, (vi) rerouting the metabolic flow, (vii) formation of compatible solutes, (viii) export of 'overflow' metabolites or (ix) going into dormancy. We propose to quantify each of these adaptations in a systems biology approach. The issue should be most acute for thermophiles. This research programme will therefore study the central carbohydrate metabolism (CCM) of Sulfolobus and its regulation under temperature variation. The archaeal CCM has pathways and enzymes that differ from their bacterial or eukaryotic counterparts. Details of regulation and energetic of the CCM will be revealed while we will assemble the data required for integration of genomic, transcriptomic, proteomic, metabolomic, kinetic and biochemical information on the effects of temperature changes. The focus shall be on a part of the CCM, i.e. the unusual, branched Entner-Doudoroff (ED) pathway for glucose and galactose uptake and catabolism to pyruvate. The required data accuracy will be achieved by combining the expertise of a number of specialized laboratories in different European countries. Our long term goal is to use our proposed Analysis, Modelling and Experimental Design Platform as a nucleus for building a sufficiently precise replica for this part of the living cell ('a Silicon Cell') to enable computation of life, particularly its robustness to changes in temperature, at the system level.

Summary

Most living organisms are rather robust vis-à-vis a great number of changes in their environment. Mammals and birds achieve much of this robustness by having different organs and cells process different tasks in a highly coordinated fashion. Unicellular microrganisms may derive much of their robustness from intracellular networks. This is somewhat understood for two of the three domains of Life, but not yet at all for the most recently discovered life form, i.e. Archaea. Yet it is these Archaea that are subject to the most extreme challenges from the environment as some of them live in hyperthermal vents or in salt and are subject to sudden and extreme variations. Only recently, the science has been developed that analyzes how intracellular networks generate important properties that are absent from the components of those networks. In much the same way groups of people can be much more effective than the same number of people all operating individually.) This 'Systems Biology' will here be deployed and tuned to analyze mechanisms of robustness in one of the most extreme Archae that is presently amenable to such studies, i.e. Sulfolobus solfataricus. The research program brings together much of the most appropriate expertise from four European countries. It will focus in the robustness of perhaps the most vital and massive biochemical functions of all organisms, i.e. their main carbon and energy metabolism. It wll look at perhaps the most pervasive perturbations cells of microorganisms experience, i.e. variations in temperature. A computer 'replica' of central carbon and energy metabolism will be made. Both in well-coordinated experiments and in the computer replica the various mechanisms through which the organisms achieve robustness will be identified and quantified. The extent, to which the mechanisms can stand in for each other should one of them be eliminated, will be determined. This new aspect of Systems Biology will suggest new ways to design drugs against parasytic cells, where adaptation of those cells is taken into account. The understanding achieved will also help develop these organisms as perhaps ideal biological factories for chemicals, including energy-fuel that needs to be harvested from extreme environments.

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	Microbiology, Systems Biology
Research Priority	X – Research Priority information not available
Research Initiative	Systems Biology of Microorganisms (SysMo) [2007-2008]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/F003420/1 Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation (P-N-01-09-23)

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