Award details

Systems analysis of guard cell oscillatory mechanics in stomatal dynamics

Reference	BB/F001673/1
Principal Investigator / Supervisor	Professor Michael Blatt
Co-Investigators / Co-Supervisors	Dr Tianhai Tian
Institution	University of Glasgow
Department	School of Life Sciences
Funding type	Research
Value (£)	408,450
Status	Completed
Type	Research Grant
Start date	15/03/2008
End date	14/03/2011
Duration	36 months

Abstract

Stomata of plant leaves play a vital role in regulating CO2 exchange for photosynthesis while minimising transpirational water loss to the atmosphere.We know a great deal about stomatal movements between the extremes in pore aperture and about the mechanisms that drive these movements at the level of the guard cell in vitro in epidermal peels and protoplasts. By contrast, our knowledge is remarkably poor of the mechanisms that give rise to the dynamic continuum of apertures normally observed in vivo in the leaf. This project seeks to bridge these gaps in knowledge to understand how stomata achieve a dynamic range of apertures in the face of environmental challenge. We propose a systems modelling approach in conjunction with experimental analysis of the oscillatory behaviour of guard cells in epidermal peels and in situ in the leaf. The project will follow two lines of enquiry. (1) We will develop a software platform for quantitative mathematical modelling of guard cell membrane transport and homeostasis. We will draw on the wealth of detailed kinetic information for guard cells to fully-constrain parameters and generate models with true predictive power and experimentally-testable outputs. (2) We will utilise imaging, electrophysiology and related techniques to quantify the oscillatory behaviours of guard cells, both to bridge the gap between in vitro preparations and stomata function in the leaf, and to dissect the underlying mechanics and function(s) of these oscillations. These studies will also serve as a test-bed for experimental analysis of model predictions and for model validation. We anticipate the results to yield new and exciting insights into the behaviour of guard cells and stomata / much as previous modelling efforts have provided hitherto unexpected (indeed, counter-intuitive) insights into cellular homeostasis and human disease / and we anticipate the work to open entirely new dimensions in applications to agriculture and crop development.

Summary

Stomata in the epidermis of plant leaves play a vital role in regulating CO2 exchange for photosynthesis while minimising transpirational water loss between the inner leaf air space and the atmosphere. Guard cells surrounding the stomata take up inorganic solutes and water, increasing in volume to open the stomatal pore when CO2 in the leaf is depleted; and they lose solutes and water, decreasing in volume to close the stomatal pore and conserve leaf water under stress, in the dark and when CO2 is high. We know a great deal about the mechanisms that drive stomatal movements between the extremes in pore aperture. By contrast, our knowledge is remarkably poor of the mechanisms that give rise to the dynamic continuum of apertures normally observed in the field, much less how such fine-tuning is regulated. This gap in knowledge can be seen, for example, in the focus of past efforts in quantitative modelling. Stomatal characteristics underpin models for transpiration and plant water use efficiency that have proven successful in reproducing and predicting transpirational behaviours at the plant and community levels. However all of these models reflect a 'top-down' approach and consider guard cell mechanics as a 'black box', subsuming these processes within a few empirical parameters, hydraulic pathways and conductances. There are very few models that have been developed 'bottom-up' from the properties of the guard cells themselves, despite the wealth of knowledge we have for guard cell transport and signalling, and none that are sufficiently generalised to be widely applicable in predicting stomatal behaviour. A further complication is that much of our knowledge at the cellular level is based on in vitro studies with guard cells in epidermal peels or isolated as protoplasts. We need to bridge these gaps in our knowledge and to understand how stomata compensate dynamically in the face of real environmental challenges. Studies over the past 15 years have yielded severalimportant clues to the mechanisms behind stomatal dynamics. The clues point to oscillations of the guard cell membrane between two quasi-stable states that control and balance osmotic fluxes. This postulate finds support in well-documented observations that stomatal apertures also oscillate and can be driven experimentally under defined conditions. Indeed, such a 'time-averaging' mechanism has already been predicted from a systems analysis of guard cell ion transport, albeit using a mathematical model with significant parameter limitations. These several lines of evidence need now to be drawn together and subject to rigorous experimental testing in order to address a number of key issues. We need to know whether more comprehensive mathematical models for guard cell transport / incorporating, for example, known regulatory properties for the major ion transporters / are able to return the full range of observed behaviours in aperture and voltage, and to predict novel ones. We want to know how these behaviours are underpinned by the dynamics of guard cell ion fluxes and osmotic contents. Finally, we want to test whether experimental manipulations of the relevant guard cell parameters can be shown to yield well-defined and predictable changes in stomatal behaviour. We propose here to develop this line of enquiry jointly through systems kinetic modelling to derive quantitative and testable predictions and through experimental analysis and validation. Our knowledge of guard cell transport and homeostasis is now sufficiently well-developed to make an approach of this kind a readily achievable goal. We fully expect answers to the questions we pose to yield new and exciting insights into the behaviour of stomata and to open entirely new dimensions to practical applications in agriculture and crop development.

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	Plant Science, Systems Biology, Technology and Methods Development
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/F001177/1 Systems analysis of guard cell oscillatory mechanics in stomatal dynamics

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search