Award details

Integrated analysis of stem cell function in plant growth and development

Reference	BB/E024858/2
Principal Investigator / Supervisor	Professor James Murray
Co-Investigators / Co-Supervisors
Institution	Cardiff University
Department	School of Biosciences
Funding type	Research
Value (£)	310,368
Status	Completed
Type	Research Grant
Start date	01/01/2009
End date	31/10/2010
Duration	22 months

Abstract

Stem cells are essential to the growth and development of plants and provide the ultimate origin of all agriculture and forestry. Although some key genes required for the establishment and maintenance of stem cells are identified, we lack information on the networks governing cell differentiation and cell cycle of different stem cell populations, on how these mechanisms determine common and specific behaviors of the different stem cell groups and how they integrate stem cell activity with changing environmental conditions. This proposal integrates the work of world-leading labs that perform key research on stem cell populations of the shoot, root and vascular meristems, cell cycle control, growth modeling and image analysis. Europe has a global lead in plant stem cell research and cell division control, and this proposal will integrate the research of the different labs involved, creating new synergies and substantial added value. New tools and strategies combining genomics, reverse genetics, smart genetic screens, and novel cell biology will be exploited to address the key issue of how specification of stem cell regions by transcription factors translates to cellular mechanisms for division and differentiation and identify common and distinct regulatory networks in different stem cell populations. [from ERA-PG project]

Summary

Stem cells are of central importance in the development of both plants and animals, since they are a self-maintaining reservoir of unspecialised cells that provide the precursor cells for tissue and organ formation. Stem cells have the ability to maintain temselves and also to produce daughter cells with different characteristics (called 'differentiated' cells). The maintenance of stem cells is therefore crucial for all multicellular organisms and is of outstanding significance for regenerative biology in medicine and agriculture. Given the life-long importance of stem cells, they are tucked safely from harm's way, in so-called stem cell niches that provide a microenvironment promoting self-renewal and inhibiting cell differentiation into different cell types. Plant stem cell niches are located in meristems at root and shoot tips, and are pivotal to the production of new organs and tissues throughout the plant life cycle that in some species can span several thousand years. In woody plants a further specialised cylindrical meristem within the stem, the cambium, is of particular importance in secondary thickening that results in production of woody material, and this also contains stem cells. In this proposal, we seek to use microarray analysis to identify the genes that control behaviour of these different populations of stem cells. Microarrays allow simultaneous measurement of the expression of all genes in a sample. We will combine this with advanced cell sorting technologies and techniques to increase the number of stem cells by transgenic regulation, and this will allow us to identify common and distinct mechanisms that control the proliferation of different stem cells and whether and when they differentiate to give rise to different cell types in their respective tissues. In plants, these signals that specify and maintain stem cells and the genes involved are poorly understood. We also seek to understand how stem cells respond to cues from the environment. We propose a European network composed of the world leading groups involved in understanding (1) root stem cells [Ben Scheres, Utrecht, Netherlands], (2) shoot stem cells (Thomas Laux, Freiburg, Germany], (3) the cambial stem cells (Yka Helariutta, Helsinki, Finland), together with the group of Jim Murray (Cambridge, UK) who are experts in the control of cell division, and Aurelio Camphilo (Porto, Portugal), experts in image analysis of growing plant tissue.

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	European Research Area Network in Plant Genomics (ERA-PG) [2006-2009]
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/E024858/1 Integrated analysis of stem cell function in plant growth and development

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