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Molecular analysis of actin-based motility of Burkholderia spp.

Reference	BB/E021212/1
Principal Investigator / Supervisor	Professor Mark Stevens
Co-Investigators / Co-Supervisors	Dr Joanne Stevens
Institution	The Pirbright Institute
Department	Div of Microbiology Compton
Funding type	Research
Value (£)	400,737
Status	Completed
Type	Research Grant
Start date	01/10/2007
End date	30/09/2010
Duration	36 months

Abstract

Control of actin assembly is essential for a plethora of cellular processes. In addition, subversion of actin dynamics by facultative intracellular pathogens facilitates their entry and exit from host cells, with key implications for pathogenesis. The study of bacterial and viral actin nucleators has yielded fascinating insights into the composition and regulation of protein complexes at sites of actin assembly and provided cell-free models to study lamellipodia and filopodia formation. With support from the BCB Committee, we recently identified a family of novel Type V secreted proline-rich actin-binding proteins that mediate actin-based motility of Burkholderia spp. by stimulating the continuous polymerisation of actin at a single pole. The structure, function and interactions of B. pseudomallei BimA and its orthologues are not understood. Here we propose to identify BimA domains required for actin binding, polymerisation and motility by site-directed mutagenesis and examine if its activity is regulated by phosphorylation and/or glycosylation as predicted. The protein complex assembled by BimA will be analysed and interacting partners localised to B. pseudomallei-induced actin tails in vivo and added to in vitro reconstitution assays. The composition of protein complexes assembled by BimA orthologues from related species will also be assessed to determine if they stimulate actin-based motility by distinct mechanisms. These studies will simultaneously dissect the mode of action of key microbial virulence factors and produce important new knowledge on the regulation of cellular actin dynamics.

Summary

Burkholderia pseudomallei is a bacterial pathogen that causes melioidosis, a severe and emerging disease of animals and humans that is endemic in the subtropics. In common with some members of the genera Listeria, Shigella, Rickettsia and Mycobacterium, B. pseudomallei has evolved the remarkable ability to enter host cells and propel itself within and between cells by hijacking the cellular machinery that assembles actin, a key structural component of the cell skeleton. In all cases so far examined, the ability of these organisms to fire themselves into adjacent cells on actin 'rockets' is required to cause disease, presumably as it aids cell-to-cell spread of the bacteria while evading host immune surveillance. The molecular basis of the actin-dependent movement of Listeria and Shigella have been extensively studied, partly to understand how the organisms cause disease, but also because it provides insights into how actin assembly is orchestrated and regulated inside cells. Actin is one of the most abundant proteins in our cells and control of actin assembly is essential for a multitude of processes, including cell migration during embryo development, wound healing and invasive cancer, as well as the movement and ability of immune cells to fight infection. With BBSRC funding we recently identified a bacterial factor that is required for actin-based motility of B. pseudomallei (BimA), as well as similar functional proteins in related Burkholderia species. Though we have established that these factors bind actin and promote its assembly in the test tube and inside host cells, the mechanism by which they do this is not understood. In this proposal we seek to: 1. Identify regions of the BimA protein that are required for actin binding and assembly. 2. Determine whether modifications of BimA by addition of phosphate and/or sugar groups occur inside cells, and investigate their functional consequences. 3. Examine the complex assembled by BimA at the bacterialsurface and the protein-protein interactions that occur within it. 4. Detect host cell proteins in B. pseudomallei-induced actin tails inside cells and determine their functional relevance. 5. Determine if BimA proteins from different Burkholderia species promote the formation of complexes with differing compositions. Taken together, we believe that these experiments will explain the mode of action of key microbial virulence factors and produce important new knowledge on the mechanisms by which actin dynamics are controlled in our cells.

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	Animal Health, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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