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Investigating the role of microRNA in translational control in Xenopus oocytes

Reference	BB/E016316/1
Principal Investigator / Supervisor	Dr Nancy Standart
Co-Investigators / Co-Supervisors	Professor Eric Miska
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	553,288
Status	Completed
Type	Research Grant
Start date	01/08/2007
End date	31/01/2011
Duration	42 months

Abstract

Early development is regulated by temporal and spatial changes in translation of pre-existing mRNAs. The cytoplasmic polyadenylation element binding protein (CPEB) is a critical regulator of translation in all metazoa. CPEB binds CPE elements in the 3' UTR of specific mRNAs, and represses their translation in oocytes, and activates their translation by polyadenylation in eggs. Towards understanding the mechanism of CPEB action, we have identifed 4 polypeptides that interact with CPEB in Xenopus oocytes: the RNA helicase Xp54, P100/Pat1, 4E-T and eIF4E, the cap-binding protein. These proteins have been implicated in translational regulation and/or early development and, intriguigingly, are found in distinct cytoplasmic bodies, in other model systems. In mammalian cells, untranslated RNA (which may be degraded), including mRNAs translationally repressed by microRNAs, collect within or nearby to P bodies. The results from several labs thus attest to a highly unusual convergence of conserved factors and mechanisms in yeast, oocytes and mammalian cells / unifying RNA decay, translational repression and microRNA-mediated regulation of gene expression. We propose to combine the expertise of the Standart lab in the biochemistry of maternal RNA regulation with the microRNA expertise of the Miska group to dissect the roles of microRNA in the Xenopus oocyte. In this cell, specific RNAs are tightly regulated at the level of translation to control the cell cycle. Our interrelated aims are to i) identify CPEB-associated mRNAs, maternal miRNA and CPEB-associated miRNA, ii) examine whether miRNAs repress translation in oocytes, iii) investigate the role of maternal miRNP components in early development, and iv) localise CPEB complex proteins, targets and miRNP components in oocytes. Knowledge of the factors and mechanisms in oocytes will have critical implications not only for our understanding of gene expression in the soma, but also in neurons, and in cancer cells.

Summary

One of the key questions which fascinates biologists is how a complex organism arises from a single cell, the egg. Development is driven by controlling when and where particular genes are used. While it used to be thought that most control of gene expression occurred when DNA is copied to give RNA, it is now becoming apparent that, during development, control more often occurs by regulating when and where previously-synthesised RNA gene copies are used to make protein. Our lab has been studying a critical regulator of protein synthesis in early development called CPEB (standing for cytoplasmic polyadenylation element binding protein ), which is conserved in structure and function in a wide variety of organisms. We investigate CPEB in the eggs of a frog, Xenopus laevis, an important organism for studying vertebrate development, and highly suitable for biochemical experiments. This protein regulates the levels of other proteins that are crucial for early embryonic development by binding to their mRNAs. We want to know how CPEB regulates protein synthesis. Recently we showed that CPEB co-purifies with another conserved protein, Xp54, which is an RNA helicase and may thus influence the conformation of bound mRNA or proteins. Additional members of the complex include X4E-T, and P100 proteins, which are known to be important in regulating protein levels in yeast, fly and human cells; the conservation implying their importance. Surprisingly, in yeast and man, CPEB complex components are present in distinct granules in the cytoplasm (so called P Bodies), places where untranslated mRNA resides, and where it may be degraded, and which also contain a new class of small RNAs, microRNA, which regulate protein synthesis. We have yet to identify all CPEB-associated mRNAs and miRNAs. We will use microRNA arrays to examine which of these small RNAs are present in oocytes, and in the CPEB complex, and study the role of miRNA in regulating protein synthesis in oocytes.Knowledge of the factors and mechanisms in oocytes will have critical implications not only for our understanding of gene expression in yeast and human somatic cells, but also in neurons, where CPEB regulates translation and in cancer cells, where some miRNAs are significantly altered in abundance.

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	Neuroscience and Behaviour
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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