Award details

2-Photon Imaging: From Polymeric Materials to Engineered Tissues

Reference	BB/E012981/1
Principal Investigator / Supervisor	Professor John Haycock
Co-Investigators / Co-Supervisors	Professor Steven Armes, Professor Giuseppe Battaglia, Professor Ian Brook, Professor Mark Geoghegan, Professor Paul Hatton, Krebs Professor of B Christopher Hunter, Professor Richard Jones, Professor Graham Leggett, Professor Sheila MacNeil, Professor Stephen Rimmer, Professor Anthony Ryan, Professor Julia Weinstein, Professor Nicholas Williams
Institution	University of Sheffield
Department	Materials Science and Engineering
Funding type	Research
Value (£)	241,039
Status	Completed
Type	Research Grant
Start date	31/01/2008
End date	30/01/2009
Duration	12 months

Abstract

The current proposal is for the single purchase of a titanium-sapphire femtosecond pulsed NIR laser and a beam splitter to add to two existing confocal microscopes that are currently located in different departments. One system is inverted and equipped for fluorescence correlation spectroscopy (FCS) and the other is upright. Relocation of these will enable us to establish a single-site multiphoton / FCS imaging facility within the Kroto Research Institute at Sheffield University. This proposal outlines a number of highly developed and interconnected themes from 14 applicants that extend across the interface between the physical and life sciences, spanning polymeric biomaterials to tissue engineering, and justifies why a parallel two-photon confocal microscope facility with FCS is needed to develop our research programmes. This is most effectively answered by requesting money for a central multi-user facility that will have extensive multidisciplinary use and be based in a dedicated institute established for research across the disciplines.

Summary

Two-photon microscopy is a leading-edge imaging technology and a powerful research tool that combines long wavelength excitation and laser scanning microscopy. Of importance to our work it can enable capture of high resolution three dimensional images of living cells within 3D constructs as well as in-depth penetration of specimens tagged with very specific fluorophores. This technology is now becoming a method of choice for the dynamic imaging of biological and polymeric systems, not otherwise possible by other optical approaches and therefore will underpin a broad number of research programmes in biomaterials and tissue engineering.

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	Industrial Biotechnology, Regenerative Biology
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2006 (RE6) [2006]
Funding Scheme	X – not Funded via a specific Funding Scheme

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