Award details

Time-based high-content screening

Reference	BB/E012574/1
Principal Investigator / Supervisor	Professor Rachel Errington
Co-Investigators / Co-Supervisors	Emeritus Professor Paul Smith
Institution	Cardiff University
Department	School of Medicine
Funding type	Research
Value (£)	239,468
Status	Completed
Type	Research Grant
Start date	01/05/2007
End date	30/04/2008
Duration	12 months

Abstract

We have identified a need for cell-based analysis to keep pace with other high-through-put orientated applications such as microarray technologies and proteomics. Our overall strategy is to acquire an image screening capacity capable of high-resolution imaging of live cells. The requirement to undertake systematic kinetic imaging assays for functional genomics and cell biology screening in Cardiff is significant. The purpose of the research equipment bid is to directly address critical issues of bottlenecks in data acquisition, handling and analysis which have thus far restricted high-content approaches. Therefore the overall aim is to establish five integrated screening platforms, which include advanced multiplex acquisition modules, data informatics and data mining tools. The proposed equipment bid will not only greatly augment the imaging capabilities within Cardiff University, but also will enhance our understanding of the best practice for use of such instrumentation for academic research and systems biology.

Summary

Microscopy and cellular imaging has hitherto been amenable to small-scale experimental samples. New approaches to cell-based analysis are required to meet the demanding needs of cell-biology, genetics and experimental therapeutics. At the core is the need to convert images into numbers, this is a significant challenge which would greatly enhance the ability of the biologist to effectively interrogate and interpret complex data. To achieve large-scale imaging experiments we need to address critical issues of data bottlenecks. High-throughput screening instrumentation has been available in the commercial sector for the last five years, therefore the objective is to bring these capabilities to academia. Such equipment not only greatly augments research capacity but provides new opportunities taking a systems approach to cell-biology.

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2006 (RE6) [2006]
Funding Scheme	X – not Funded via a specific Funding Scheme

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