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Optimising wheat grain shape for improved processing quality

ReferenceBB/E007139/1
Principal Investigator / Supervisor Professor Robbie Waugh
Co-Investigators /
Co-Supervisors		 Dr Arnis Druka, Dr Brian Forster, Dr William Thomas
Institution The James Hutton Institute
DepartmentGenome Dynamics
Funding typeResearch
Value (£) 263,423
StatusCompleted
TypeResearch Grant
Start date 01/03/2007
End date 30/04/2010
Duration38 months

Abstract

This programme aims to optimise end user quality traits in wheat and barley. Milling yield is dependent on size and shape of grains and on the proportion of endosperm to the other tissues in the grain. The recessed ventral groove of wheat is a particular obstacle to the efficient extraction of the white flour and the adoption of new de-branning technology in UK mills has further increased the importance of crease morphology. For barley, a short, fat grain is desirable to assist water uptake and rapid starch mobilisation during malting. We will use forward and reverse genetics approaches combined with knowledge of seed development in model systems to improve our understanding of grain size and shape. This programme involves the major laboratories presently engaged in research in grain and seed development in the UK, together with a leading group working on milling technology allowing the benefits of altered grain shape to milling yield and malting quality to be assessed. We have shown that UK wheat varieties differ in grain morphology and will extend our analysis to broader germplasm including T. aestivum sphaerococcum which has rounded grains and a reduced crease. Introgression of the 'sphaerococcum' phenotype into elite UK material will allow the effects on de-branning and milling yield to be determined. The segregation of QTLs for grain shape will be studied in different populations to identify linked markers. We will also map and identify genes that may be controlling a similar (globosum) phenotype in barley. Our work in the model species Arabidopsis and maize is resolving the processes and genes that are involved in cell proliferation and differentiation in the endosperm. We hypothesise that cereal orthologues of these genes may underlie QTLs for grain size and shape. The expression of selected candidates will be altered in transgenic wheat or through TILLING in barley and an assessment of grain morphology and quality traits compared to parental lines.

Summary

This programme aims to optimise end user quality traits in wheat and barley. Milling yield is dependent on size and shape of grains and on the proportion of endosperm to the other tissues in the grain. The recessed ventral groove of wheat is a particular obstacle to the efficient extraction of the white flour and the adoption of new de-branning technology in UK mills has further increased the importance of crease morphology. For barley, a short, fat grain is desirable to assist water uptake and rapid starch mobilisation during malting. We will use forward and reverse genetics approaches combined with knowledge of seed development in model systems to improve our understanding of grain size and shape. This programme involves the major laboratories presently engaged in research in grain and seed development in the UK, together with a leading group working on milling technology allowing the benefits of altered grain shape to milling yield and malting quality to be assessed. We have shown that UK wheat varieties differ in grain morphology and will extend our analysis to broader germplasm including T. aestivum sphaerococcum which has rounded grains and a reduced crease. Introgression of the 'sphaerococcum' phenotype into elite UK material will allow the effects on de-branning and milling yield to be determined. The segregation of QTLs for grain shape will be studied in different populations to identify linked markers. We will also map and identify genes that may be controlling a similar (globosum) phenotype in barley. Our work in the model species Arabidopsis and maize is resolving the processes and genes that are involved in cell proliferation and differentiation in the endosperm. We hypothesise that cereal orthologues of these genes may underlie QTLs for grain size and shape. The expression of selected candidates will be altered in transgenic wheat or through TILLING in barley and an assessment of grain morphology and quality traits compared to parental lines.
Committee Closed Committee - Agri-food (AF)
Research TopicsCrop Science, Plant Science
Research PriorityX – Research Priority information not available
Research Initiative Crop Science Initiative (CSI) [2006]
Funding SchemeX – not Funded via a specific Funding Scheme
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