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Comparative genomics approaches to the analysis of temperate grasses

Reference	BB/E004725/1
Principal Investigator / Supervisor	Professor Michael Bevan
Co-Investigators / Co-Supervisors	Dr Martin Trick
Institution	John Innes Centre
Department	Cell and Develop Biology
Funding type	Research
Value (£)	471,750
Status	Completed
Type	Research Grant
Start date	19/07/2007
End date	18/07/2010
Duration	36 months

Abstract

We aim to establish a comparative genomics approach to the members of the Pooideae subfamily of grasses that includes wheat and barley, the most important crop species in the UK and Europe. Brachypodium is a member of this family but it has a compact genome of about 300 Mb, a little smaller than that of rice, compared to the 16,000 Mb hexaploid genome of wheat. There is a much greater conservation of gene sequence and a general conservation of gene order between wheat and Brachypodium than between wheat and rice. Comparison of gene order and sequence in wheat, barley, rice and Brachypodium has been used to assemble and interpret a large region of wheat chromosome 5B containing the Ph1 locus controlling chromosome pairing and to characterise the Hardness loci in bread wheat and its progenitors. Probes isolated from Brachypodium genes invariably give unambiguous hybridisation signals when used on wheat BAC filters and southern blots and permitted large physical maps of wheat to be made rapidly. The close similarity of gene sequences permitted their exon-intron structures to be defined. A physical map of the Brachypodium distachyon genome will be assembled from end-sequenced BAC clones for use in whole-genome comparative genomic studies in wheat and barley. The physical map of BACs can be made very efficiently by initial alignment of BAC end sequence to the rice genome to generate shorter-scale assemblies that will be verified by correlation to the Brachypodium genetic map. The physical map provides a very high density of sequences that can be used to identify wheat ESTs for aligning wheat BAC contigs and for designing sets of very high density markers. It therefore provides a very cost-effective resource for gene identification in bread wheat and its progenitors, barley and forage grasses.

Summary

The grass family of plants provides about 60% of the global food supply for humans and our domesticated animals. A deeper understanding of the biology of this group of plants, which includes rice, wheat, barley, forage grasses, maize and sorghum will greatly improve our ability to breed better crops- for example to improve nutrition, disease resistance and capacity to endure environmental stresses such as drought. Genomics, which identifies all of the genes in the organism (its genome) is a powerful tool in biology as it permit researchers to identify genes and useful genetic variation. However the main UK crops in the grass family, wheat, barley and forage grasses have the largest and most complex genomes known- even larger and more complex that the human genome, and consequently identifying genes is currently complex, uncertain and time consuming. This is a major barrier for scientists and plant breeders. In this proposal we describe a comparative genomics strategy that will provide direct and cost-effective ways accelerating of constructing physical maps of the wheat and barley genome. A promising approach to tackling the problem of these large and complex genomes is to compare them with simpler and smaller genomes. This is an excellent strategy for grasses as their genomes are more similar to each other than most other plant groups, and mainly differ by the expansion of repeated sequences (so called 'junk DNA') between genes. One member of the grass family, rice, has a completely sequenced genome- that is we know the identity and order of nearly all of the rice genes and the sequences between them. In principle the rice genome sequence can be used to identify wheat genes and order these, but in practice this does not work effectively enough for systematic analysis as the rice and e.g. wheat genomes have diverged substantially over the 70 million years (myr) separating these plants. Recent work to identify genes in wheat and barley has taken a similar approach but used the small genome of Brachypodium, a closer relative of wheat, barley and forage grasses. Being closely related, its genes are very similar and gene order is generally more conserved, so it can be used to interpret the wheat genome directly. Comparison of wheat, rice and Brachypodium genomes over small scales has shown that the three-way comparison is exceptionally powerful for defining gene structures and gene order in wheat and barley. We aim to make a bridge between the rice genome and the genomes of wheat, barley and forage grasses by making a physical map of a well characterised single-seed descent line of Brachypodium distachyon. This physical map will comprise an ordered set of large cloned fragments of DNA covering about 80-90% of the genome. This physical map will permit the effective and rapid identification of extensive related regions of the genomes of closely related species including wheat, barley and forage grasses that are major UK crops. In the case of barley, which has a well-advanced physical mapping programme, this work will help completion of an accurate BAC map. By making it easier to characterise the DNA sequence of large regions of these crop genome, scientists will be able to identify genes and genetic variation that can be used to understand many important biological processes in the most important UK crop. This knowledge will help breed the cereal crop plants of the future that are optimally adapted to their environment and with improved sustainable yield, nutrition and end-uses such as biomass and bioenergy.

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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