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Design and analysis of small molecule ligand binding to the 7TM receptor for GLP-1

Reference	BB/E003583/1
Principal Investigator / Supervisor	Dr Daniel Donnelly
Co-Investigators / Co-Supervisors	Professor Colin Fishwick, Professor Arthur Johnson
Institution	University of Leeds
Department	Institute of Membrane & Systems Biology
Funding type	Research
Value (£)	607,609
Status	Completed
Type	Research Grant
Start date	05/03/2007
End date	04/10/2010
Duration	43 months

Abstract

The hormone glucagon-like peptide-1 (GLP-1) is a critical component of the body's mechanism for controlling blood sugar levels following the ingestion of a meal. It is released into the bloodstream from intestinal L cells and targets receptors on the surface of pancreatic beta cells. The receptor is also activated by a peptide called exendin-4 (EX-4), originally discovered in the venom of a poisonous lizard and now used to treat diabetes mellitus. The GLP-1 receptor (GLP-1R) is a 'Family B' G protein-coupled receptor, characterised by an extracellular N-domain of approximately 120 residues and a 'core domain' consisting of the seven transmembrane helices and connecting loop regions. We will use a homology model of the GLP-1 receptor N-domain with SPROUT to design, synthesise and pharmacologically analyse potential non-peptidic ligands for the GLP-1 receptor.Using automated parallel synthesis, we will create small focused libraries of compounds which will be screened for their ability to inhibit 125I-EX-4(9-39) binding. Docking models for successful hits will be validated by site-directed mutagenesis. In addition, we will determine the N-domain residues which participate in binding to the C-terminal tail of EX-4 (residues 31-39). Disruption of this interaction by mutagenesis will result in receptors with normal GLP-1 affinity but reduced (>50-fold) affinity for EX-4. The successful identification of the residues responsible for this interaction will eliminate many of the false peptide-receptor docking models obtained from our theoretical studies and enable us to focus on the most likely model. Finally, we will identify the residues in the GLP-1R core-domain responsible for interacting with several non-peptide GLP-1R agonists supplied by Astra Zenica. In doing so, we will identify the activation pocket and explore whether this is shared with peptide agonist molecules.

Summary

Hormones are molecules which transmit signals from one part of the body to another. The hormone we work on is called glucagon-like peptide-1 (GLP-1 for short) and we are interested in this because it is essential for regulating blood sugar levels. GLP-1 is released into the blood stream following consumption of a meal. The main role of the hormone is to get the body ready for the upcoming rise in blood sugar levels that will inevitably result from the digestion of the food - high blood sugar levels are dangerous. The cells which release the GLP-1 hormone are in the small intestine of the gut but once released into the blood, it is transported to the surface of cells located in the pancreas. Once there, the hormone binds to specialised proteins on the exterior surface of the pancreatic B cells - these proteins are embedded in the membrane of the B cells and are called receptors. Receptors are proteins which recognise signals on the outside of cells and relay them to the cell's interior and there are many different types of receptor, each designed to detect one particular signal. In the case of our particular receptor, it will only recognise the hormone GLP-1 but will not recognise even very closely related hormones, for example glucagon. Once activated, the receptor for GLP-1 tells the B-cells that a rise in blood glucose levels is imminent. The B-cells then switch on their machinery in order to prepare to release another hormone, called insulin, which will reduce blood sugar levels to a safe level. We want to understand how the GLP-1 hormone recognises its own receptor rather than the multitude of other molecules in its environment. We also want to know how the receptor recognises a drug called exenatide which was discovered in the venom of a poisonous lizard and which is now used to treat non-insulin dependent diabetes. In addition, we want to know how synthetic compounds, very different from the peptide hormone, can bind and activate this receptor. Indeed, weplan to design and synthesise new non-peptide compounds to bind to the receptor and, in doing so, increase our understanding of this important signalling molecule. The GLP-1 receptor itself is a member of a wider family of related receptors that are critical to many other biological signalling processes and hence what we learn about this receptor can be used to aid our understanding of others.

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	Structural Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	Industrial Partnership Award (IPA)

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