Award details

Molecular analysis of gene regulators in the remarkable iron-ome of the symbiotic bacterium Rhizobium.

Reference	BB/E003400/1
Principal Investigator / Supervisor	Professor Andrew Johnston
Co-Investigators / Co-Supervisors	Professor Nicolas Le Brun, Professor Jonathan Todd
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	423,748
Status	Completed
Type	Research Grant
Start date	01/11/2006
End date	30/04/2010
Duration	42 months

Abstract

One major objective is to understand how the unusual, iron-responsive transcriptional regulator Irr functions in the symbiotic bacterium Rhizobium, relating this to the overall iron regulon in free-living forms of these bacteria. Using microarrays, we wish to identify all the Irr-regulated genes in the Rhizobium genome. We will also verify that Irr binds to the ICE cis-acting regulatory motif. A major aim is to see how Irr responds to elevated levels of iron in vivo. Good evidence suggests that Irr degrades following haem-binding and we will investigate its stability in Fe-replete conditions using anti-Irr antibody, and also follow the fate of Irr-Gfp fusion proteins, to determine if the degradation is specific for Irr. By mutagenising irr, we will identify regions and individual residues that affect its haem-dependent stability. We will also use genomic mutagenesis to explore the exciting possibility that other cellular factors are needed for Irr degradation. We will use optical and magnetic spectroscopies and bioanalytical methods to probe the Irr haem-binding site(s), to determine spin and oxidation states and redox properties, from which we will obtain a picture of the physical properties of the haem site(s). Haem-dependent changes in association state, conformational changes, and effects on protein fold stability will be probed to reveal how the binding of haem acts as a signal for degradation in the cell. We will also investigate if Irr has a Zn(II)-binding site. We also wish to build on very recent observations on Fe-responsive regulation of a gene, hemA2, which may specifically function in bacteroids in root nodules, but which is not regulated by Irr or RirA, the 'free-living' regulators. By identifying and starting to characterise the Fe-responsive regulator of hemA2, we will address the important question of how Rhizobium regulates its genes in response to iron in its symbiotic state.

Summary

Iron may or not be in the soul, but it is certainly in the soma, as a major player in the functioning of all living cells. It is needed to transport oxygen in the blood, to help us cope with many different toxins and for a thousand and one other essential biochemical functions. This is because iron has special chemical properties that can drive all sorts of oxidation and other energy-generating steps, and so it occurs in many enzymes that catalyse these sorts of reactions. However, an excess of iron within cells can be very harmful, since its same 'redox' properties can also generate some very nasty substances. These so-called 'radicals' can damage the DNA, the lipids and the proteins in the cells. It is therefore crucial that living things control their amounts of iron to just the right level. We are studying how this so-called 'homeostasis' is achieved in a bacterium called Rhizobium, whose main claim to fame is that it forms nitrogen-fixing nodules on the roots of legume plants. These include many familiar crops, such as peas, beans and clover and, because of their symbiosis with the Rhizobium, they are grown in soils that have no need for energy-expensive nitrogenous fertilizer. These rhizobia live in two very different environments - most of the time they have to struggle along in the soil, and, in competition with all the other bugs and beasties, they have to grasp the rather scarce iron as best they can. Indeed Rhizobium has more methods for grabbing iron from their surrounds than almost any other known living thing. However, when the lucky few individual Rhizobium cells get into the root nodules, they live in luxury, fed, watered, pampered and protected by the host plant. But iron is still important here, since many of the proteins in the nodule, including the enzyme nitrogenase that drives the nitrogen fixation reaction, contain iron. We have been studying how Rhizobium obtains its iron and how it responds to it in their free-living state. This has shown that these bacteria use methods that are totally different from those that have been described in many other bacteria, including that genetic superstar, Escherichia coli. In brief, it seems that the Rhizobium are rather sophisticated since they switch their genes on and off in response to iron in two important forms (as iron-sulphur clusters and haem), rather than in response to the free metal itself, as occurs in E. coli. We believe that similar regulatory circuitry in response to iron may operate in many of Rhizobium's close relatives. These include some bacterial pathogens, including the potential bio-terror agent Brucella, and other genera of medical, environmental or biotechnological importance. In this new project we want to investigate the molecular mechanism of one of the key players in this unusual regulatory process / the one that senses and responds to iron in the form of haem. It seems that the ways in which the Rhizobium responds to iron when in the soil are very different to that when it is in the root nodule. Up to now, understanding the latter has been elusive, but we now have the opportunity / we think / to get our hands on a regulator that operates in the nodules. We plan to identify and start to characterise this new actor in what is turning out to be an intriguing story.

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Microbiology, Soil Science, Structural Biology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search