Award details

Accumulation of PrP amyloid in vivo that is not infectious

Reference	BB/E002900/3
Principal Investigator / Supervisor	Dr Rona Barron
Co-Investigators / Co-Supervisors	Dr Andrew Gill
Institution	University of Edinburgh
Department	College of Medicine and Veterinary Medic
Funding type	Research
Value (£)	424,096
Status	Completed
Type	Research Grant
Start date	01/05/2008
End date	28/02/2011
Duration	34 months

Abstract

PrP amyloid is a feature of some but not all prion diseases, however its role in the disease process and association with infectivity are unknown. Recent conflicting experiments have suggested that PrP amyloid may both be the infectious form of PrP and an uninfectious inert aggregate of smaller infectious subfibrillar particles. It is essential to determine exactly what role PrP amyloid plays in disease to validate its accuracy as a diagnostic marker, and as a target for therapy. If amyloid is an inert aggregate, therapies which disrupt amyloid may instead enhance disease by producing more infectious particles. The aims of this proposal are to determine whether the production of amyloid can be induced in brain tissue in the absence of TSE infectivity, and whether amyloid is an inert, non-infectious aggregation of PrP, or a protective mechanism by which the host sequesters the more infectious oligomeric forms of PrP as inert fibrils. We aim to assess these issues using a unique in vivo model in which we have identified PrP amyloid plaques in the absence of clinical disease and infectivity after inoculation with material from an atypical human prion disease. We will determine whether amyloid can be induced by non-TSE disease inoculum by injecting mice with beta-folded isoforms of recombinant 101L PrP and PrP-A. These mice will be monitored for signs of TSE disease, and analysed for presence of PrP amyloid by IHC and thioflavin-s fluorescence. Tissue homogenates will be bioassayed to establish presence of infectivity. We will also attempt to disaggregate the amyloid deposits and determine whether this results in an increase in levels of infectivity. These experiments will determine whether beta-folded forms of PrP can seed the formation of amyloid in the brain, and whether such forms of PrP are infectious. These data will signify whether tissues containing only PrP amyloid should be considered infectious, and whether amyloid is a suitable target for TSE therapeutics.

Summary

The Transmissible Spongiform Encephalopathy (TSE) diseases are a group of fatal neurodegenerative diseases which include scrapie in sheep, BSE in cattle and CJD in humans. TSE diseases (also known as prion diseases) differ from other neurodegenerative disorders such as Alzheimer's disease, due to their infectious nature. Instead of an conventional infectious agent such as a bacterium or virus the TSE infectious agent (the prion) is thought to be a misfolded form of a host protein (PrP). It has been hypothesised that the abnormally folded form of the protein (PrPSc) is able to bind to the normal protein which is found in brain tissue of all mammals, and convert it into the abnormal form. PrPSc accumulates as disease progresses, and may cause the death of neurons in the brain. PrPSc is usually found in infected tissue, and can be identified microscopically by the presence of abnormal protein aggregates in sections of brain tissue, or by its resistance to digestion with proteases on immunoblotting. PrPSc co-purifies with the TSE infectious agent, and correlates with the level of infectivity present. PrPSc was therefore thought to be the sole component of the 'prion', and is currently the only diagnostic marker used for TSE disease testing. PrPSc can exist as either diffuse deposits or large amyloid aggregates in tissue, but the role of each form in disease is unknown. Conflicting studies have suggested both an infectious and a protective role for PrP amyloid in TSE disease. In addition, other experiments have shown that PrPSc is not always present in infectious tissue. These findings raise serious questions about the suitability of PrPSc as the only available diagnostic marker, and it is important for both accurate disease diagnosis and the development of new therapies and treatments for these currently incurable diseases that we identify exactly which form of PrP is associated with infectivity. In this proposal, we aim study the amyloid form of PrPSc and its association with the infectious agent. In our laboratory we have observed that transgenic mice inoculated with brain material from a case of atypical human prion disease do not develop clinical or pathological signs of disease, but do produce large amyloid aggregates in the brain. We have been unable to transmit disease from brain tissue of mice possessing these aggregates, indicating the absence of TSE infectious agent in these tissues. Current diagnostic methods would have identified these mice as TSE infected, yet we have shown the mice lack both disease and infectious agent. Our results support the hypothesis that PrP amyloid is not infectious, and may be formed by seeding from amyloid in the inoculum, or may be a host protective mechanism by which smaller more infectious aggregates are sequestered into an inert form. We therefore aim to identify the role of amyloid in TSE disease by inoculating transgenic mice with oligomeric and amyloid forms of recombinant PrP to determine whether we can induce amyloid formation in transgenic mice in the absence of infected tissue inoculum, and whether such amyloid forms of PrP are infectious. We also aim to disrupt these amyloid deposits to determine whether smaller fragments from the amyloid are infectious. The results from these experiments will aid in our understanding of the role of PrP amyloid in TSE disease. If amyloid is a protective mechanism by which the host controls TSE infectivity, treatments which target the disruption of such aggregates may instead enhance disease, and would therefore be undesirable. These results will also help to identify specific forms of PrP associated with TSE infectivity, leading to the development of accurate diagnostic tests with low risk of both false negative and false positive results which is important ethically when developing diagnostic assays for human prion disease.

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	Animal Health, Neuroscience and Behaviour, TSEs (transmissible spongiform encephalopathies)
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/E002900/1 Accumulation of PrP amyloid in vivo that is not infectious

BB/E002900/2 Accumulation of PrP amyloid in vivo that is not infectious

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