Award details

Enhancing the ability to undertake the molecular genetical analysis of cereals

Reference	BB/D524716/1
Principal Investigator / Supervisor	Professor Graham Moore
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	Crop Genetics
Funding type	Research
Value (£)	46,412
Status	Completed
Type	Research Grant
Start date	01/12/2005
End date	31/08/2006
Duration	9 months

Abstract

The proposal requests the purchase of a typhoon 9200 phosphorimager in order to facilitate the molecular analysis of hexaploid wheat and barley. This would enable BBSRC¿s cereal molecular genetics programmes based at John Innes Centre to remain internationally competitive. The genomes of hexaploid wheat and barley are considerably larger than the model plant species, Arabidopsis and rice. This makes undertaking the simplest of molecular approaches within these species (Southern blots and library analysis) a tedious and timing consuming exercise. However the development of a more sensitive technique than x-ray film for the detection of radiochemicals, x-ray and UV, based on storage phosphor technology, provides an opportunity to considerably facilitate the application of basic molecular genetical approaches to these crops. Our previous work has already demonstrated the utility of the phosphorimager in this regard. It enabled JIC to: generate the first RFLP map of wheat; reveal that gene order was conserved in wheat and rice genomes; provide a solution to the relationship of the major cereal genomes; and, recently, enable us to clone BBSRC major target loci in these crops, namely the flowering time locus in barley and the pairing control locus in hexaploid wheat, building on investments made in the BBSRC IGF programme. Moreover, the phosphorimager is regularly used by more than 15 research groups, many of whom undertake research on model plant systems. The request is therefore for a typhoon 9200, the next generation phosphorimager, which will be used in genetic mapping, library screening and RNA quantification in hexaploid wheat, barley, rice, Antirrhinum and Arabidopsis studies. In particular, it will be used in programmes aimed at identifying genes underpinning wheat and barley traits such as crossability, suppressors of the PH1 locus, photoperiod and vernalisation responses.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2005 (RE5) [2005]
Funding Scheme	X – not Funded via a specific Funding Scheme

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