Award details

Laser capture microdissection for improved analysis of biological process

Reference	BB/D524708/1
Principal Investigator / Supervisor	Professor Andrew Maule
Co-Investigators / Co-Supervisors	Dr Mary Byrne, Professor Giles Oldroyd
Institution	John Innes Centre
Department	Disease and Stress Biology
Funding type	Research
Value (£)	64,656
Status	Completed
Type	Research Grant
Start date	01/12/2005
End date	31/08/2006
Duration	9 months

Abstract

Modern biology seeks ever more analytical refinement to establish new knowledge and understanding of key biological processes in growth and development and adaptation to the environment. Advances in omics technologies have allowed researchers to analyse larger sample numbers for more molecular parameters in a shorter time, but until recently there has not been a corresponding increase in the sampling precision that would allow the spatial aspects of molecular events to be determined. Recent developments in laser technology have produced equipment designed to achieve laser capture microdissection (LCM). This technology uses lasers to remove and capture single cells or specific tissues that can be analysed for precise molecular events, most commonly using transcriptomics. We propose to purchase a P.A.L.M.LCM and to use it initially for three specific objectives. Objectives i) and iii) relate to resolving host responses to microbial (viruses, Rhizobium, Mycorrhizae) attack, while Objective ii) is aimed at creating a gene expression map for the cereal shoot apical meristem. All of the objectives will add significantly to the quality of scientific output from the John Innes Centre and to new knowledge to the benefit of the larger scientific community in line with the BBSRC Strategic Plan. Furthermore, the technology at JIC will add a new dimension to research planning for senior and junior researchers on the Norwich Research Park and will contribute significantly to wider research questions in the future.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2005 (RE5) [2005]
Funding Scheme	X – not Funded via a specific Funding Scheme

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