Award details

X-ray data collection equipment for the York Structural Biology Laboratory

Reference	BB/D524632/1
Principal Investigator / Supervisor	Professor Keith Wilson
Co-Investigators / Co-Supervisors	Professor Andrzej Brzozowski, Professor Gideon Davies, Professor Anthony Wilkinson
Institution	University of York
Department	Chemistry
Funding type	Research
Value (£)	121,414
Status	Completed
Type	Research Grant
Start date	01/11/2005
End date	31/10/2006
Duration	12 months

Abstract

Structural analysis of macromolecules and their interactions from both a fundamental and a therapeutic point of view, is recognised in the BBSRC Strategic Plan 2003-2008 `World Class Bioscience¿ as an important part of integrative biology. X-ray crystallography is the key technique in this field, and up to date data collection facilities in the home laboratory (i) allow novel methodological developments, (ii) permit automation and (iii) expand the range of projects that may be studied. We request funding for integrated X-ray equipment and a sample temperature controller. This equipment will underpin the laboratory¿s work on proteins and their complexes. In particular: the dissection and exploitation of protein-carbohydrate interactions including glycosyltransferases, enzymes involved in carbohydrate modification and processing, glycosidase inhibition, lectins and sugar binding domains; structural proteomics projects on Plasmodium falciparum, Bacillus anthracis and Campylobacter jejuni; nucleic acid binding proteins, especially viral portal proteins involved in DNA-packaging, and transcription/replication factors. The proposed equipment will allow us to assess the quality of a proportion of the crystals obtained in our laboratory, and to collect preliminary data on putative heavy atom derivatives and complexes. In addition, we will continue to collaborate with Oxford Diffraction Ltd (ODL) on the development of the software interface which drives the equipment, as well as the design and future development of an automatic sample changer which will allow a far greater throughput in crystal testing and assessment.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2005 (RE5) [2005]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search