Award details

Initiation of endospore formation in clostridia

Reference	BB/D522797/1
Principal Investigator / Supervisor	Professor Nigel Minton
Co-Investigators / Co-Supervisors
Institution	University of Nottingham
Department	Inst of Infections and Immunity
Funding type	Research
Value (£)	187,191
Status	Completed
Type	Research Grant
Start date	01/01/2006
End date	31/03/2009
Duration	39 months

Abstract

Endospore formation in the model organism, Bacillus subtilis, is now rather well understood. The decision to initiate spore formation is not taken lightly. It is controlled by the phosphorylation status of the Spo0A master regulator and information about the internal and external environment is conveyed to this transcription factor by a number of proteins comprising a phosphorelay. Several autophosphorylating kinases, whose phosphorylation status is controlled by unknown ligands, transfer their phosphate to the Spo0F response regulator. This may either be dephosphorylated, or it may transfer its phosphate onwards to the Spo0B response regulator phosphotransferase, which then, in turn, passes it on to Spo0A. Phosphate transfer through the phosphorelay is under complex regulation at several points, which makes the decision to sporulate sensitive to multiple inputs indicative of nutrient starvation. Using an elegant combination of site-directed mutagenesis, X-ray crystallography, and biochemical analyses, the residues forming the interaction surfaces between the various phosphorelay components have now been identified. Bacterial genome sequencing projects have shown that the clostridia (and related anaerobic spore-forming organisms) do not contain a recognisable phosphorelay. Some organisms contain a protein similar to B. subtilis Spo0B, but none of them appear to contain Spo0F. This raises an intriguing question: how is Spo0A phosphorylated in these organisms? A potential answer to this question is provided by recently acquired knowledge of protein-protein interactions in the phosphorelay. In B. subtilis, all the sensor kinases that feed into the phosphorelay show substantial sequence conservation in an alpha-helix that makes contact with Spo0F. The corresponding region of B. subtilis Spo0B (which contacts Spo0A) lacks sequence conservation in this helix, but shares conservation, instead, with the B. subtilis YufL (malate) and CitS (citrate) sensors and also, significantly, with the corresponding region of Spo0B found in some clostridia (and relatives). This suggests that the phosphorelay may have arisen during evolution (as oxygen became more abundant in the Earth¿s atmosphere) from genes resembling the YufLM CitSB sensor-regulator couples in the ancestral organism. This moreover suggests the testable hypothesis that kinase resembling B. subtilis YufL and CitS may phosphorylate Spo0A in clostridia. To test this, we will inactivate the Clostridium acetobutylicum and Clostridium botulinum kinases that resemble B. subtilis YufL CitS and determine whether either singly, or in combination, these genetic defects reduce the phosphorylation status of Spo0A, engendering phenotypes such as the loss of the ability to form spores and solvents or toxins and the loss of motility, characteristic of the inactivation of spo0A itself. There is also direct experimental evidence that sporulation of some clostridia can be stimulated by exposure to (traces of) oxygen, suggesting, perhaps, that one or more of their redox-sensitive PAS domain-containing kinases phosphorylates Spo0A. This will also be explored by systematic inactivation of the encoding genes. In the case of C. botulinum, the global effects of kinase gene inactivation will also be explored using microarray analysis. Inactivation will be achieved by gene replacement and by using antisense technology. Methods for undertaking gene replacement in clostridia have been developed in the authors laboratories and also in the laboratory of Philippe Soucaille, INSA, Toulouse, France. Gene replacement will be the method of choice in C. acetobutylicum, while in C. botulinum, the antisense approach will be favoured. To confirm these in vivo experiments, recombinant forms of the YufL CitS-like and PAS domain-containing kinases will be produced and their ability to transfer phosphate to clostridial Spo0A in vitro will be explored. Joint with BB/D001498/1.

Summary

Some bacteria are capable of producing profoundly dormant and highly resistant spores in nature, making them difficult to eradicate form hospital environments, foodstuffs and pharmaceutical preparations. The ability to form spores is shared by members of the bacterial families Bacillus and Clostridium. Whereas the former require atmospheric air for growth (aerobes), oxygen is poisonous to the latter. Thus, clostridia can only grow in the absence of oxygen and are therefore classified as anaerobes. A number of Clostridium species have achieved notoriety because they cause human disease, largely as a consequence of spore production. For example, Clostridium difficile is highly resistant to many antibiotics and causes serious problems in our hospitals, sometimes leading to mortality. Once an infection breaks out in a hospital ward the presence of spores make it very difficult to control and frequently, wards have to be closed for rigorous disinfection. Similarly, the spores of Clostridium botulinum remain a potentially serious problem in the food preservation industry because they are able to withstand high temperatures and pressures. Moreover, public concern about spore-forming organisms have been heightened post 9,11, as they pose a potential bioterrorism threat. Paradoxically, it is the ability to form spores that presents Clostridium with, perhaps, its greatest potential benefit to mankind, through the exploitation of their spores as a delivery system for treating cancer. When spores are injected into the bloodstream, the bacteria are unable to grow because normal healthy tissues contain oxygen. However, the central mass of solid tumors are devoid of oxygen. Those spores that enter a tumour are therefore able to germinate, and bring about the establishment of an actively growing population, specifically restricted to the tumour mass. This unique feature provides the opportunity to deliver therapeutic agents selectively to solid tumours, by endowing the organism used with genes able to direct the production of the desired anticancer drugs. The well-studied model organism, Bacillus subtilis, contains a group of interacting protein molecules (called a phosphorelay) responsible for sensing environmental changes as a prelude to launching the process of spore development. It was assumed that all spore-formers would possess a similar phosphorelay. Recently, the entire DNA sequence of the chromosomes of a number of Clostridium family members have been determined. To our surprise, they do not have phosphorelay proteins. The clostridia are believed to resemble the more primitive life forms that were present on Earth before our atmosphere contained oxygen. Little is known about the environmental changes that might signal the onset of spore formation in this important group of organisms and the objective of this research project, is to discover what, in the absence of a phosphorelay, triggers sporulation. Should we solve this riddle, then scientists may be able to devise strategies that interfere with spore formation by clostridia. This will reveal new ways of preventing spore formation and reducing the incidence of C. botulinum spores in foods and the spore load of C. difficile in hospital wards. It should also result in the more effective production of spore preparations for use in anticancer therapies. Joint with BB/D001498/1.

Committee	Closed Committee - Agri-food (AF)
Research Topics	Microbial Food Safety, Microbiology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/D001498/1 Initiation of endospore formation in clostridia

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