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Neural crest migration: control by interactions between the non-canonical Wnt pathway Syndecan-4 and chemokines

Reference	BB/D017521/1
Principal Investigator / Supervisor	Professor Roberto Mayor
Co-Investigators / Co-Supervisors
Institution	University College London
Department	Cell and Developmental Biology
Funding type	Research
Value (£)	304,288
Status	Completed
Type	Research Grant
Start date	01/10/2006
End date	28/02/2010
Duration	41 months

Abstract

The neural crest is a migratory population of cells that differentiate into a wide range of cell types. Most of the studies about crest migration have focused on the role of extracellular matrix, adhesion molecules and inhibitory signals. However the complex pattern of NC migration suggests that positive 'attractant' signals could also play a role. Based both on published material and on unpublished results from our laboratory, we propose here that non-canonical Wnt signalling, Syndecan-4 and the chemokine SDF-1 are necessary for neural crest migration, and that interaction between these three molecules/pathways control cell migration. Gain and loss of function experiments for each of these pathways will be performed and neural crest migration will be analyzed in Xenopus and zebrafish embryos. Specific downstream targets of these pathways will be studied as well as direct interaction between the receptors and ligands. We will perform chemotaxis assays to test whether NC cells can follow a gradient of SDF-1 and whether this event requires Syndecan-4 and non-canonical Wnt signalling.

Summary

The neural crest is a migratory cell population found in all vertebrate embryos, that generates several different cell types. In the head, these cells form specific components of the face, and in the body they generate the peripheral nervous system, skin pigment, etc. Several genes are required for the development of neural crest cells, and mutations of some of these genes in humans are responsible for certain diseases, such as Hirschprungs and Saethre-Chotzen diseases and Waardenburg-Shah syndrome. The neural crest cell forms at the border of the neural plate, and it is from here that the crest cells migrate to different parts of the embryo where they differentiate into a wide range of cell types. Because of their extraordinary ability to migrate, the neural crest cells have been called the 'explorer of the embryo'. The molecular mechanisms that control neural crest migration seem to be similar to the events that trigger metastasis in cancer cells. However, we still know almost nothing about how the migrating crest cells can find their correct destinations. In this project we will analyze the role of 3 different, apparently no related, molecules during neural crest migration. One possibility is that cells are attracted by specific molecules produced by their target sites (chemotaxis). One of the molecules may be a protein called SDF-1. This is the factor that triggers chemotaxis in white blood cells and we will test here if something similar occurs during neural crest migration. Furthermore, SDF-1 and its receptor (CXCR4) interact with another molecule called Syndecan-4. Preliminary evidence in our laboratory indicates that Syndecan-4 is also required for NC migration; thus, Syndecan-4 will be a second molecule to study during neural crest migration. Our preliminary data also indicate that Syndecan-4 interacts with another signalling pathway called non-canonical Wnt signalling. Our laboratory was the first to show that non-canonical Wnt signalling is required for neural crest migration. Taken together, it seems that all three of these molecules are involved in neural crest migration and that they interact with each other. In conclusion, we will test the idea that SDF-1/CXCR4, Syndecan-4 and the non-canonical pathway control neural crest migration and that they interact during this event. This research will teach us a lot about the molecular mechanism of neural crest migration in particular, and about cell migration in general. The principles uncovered by this work will also be relevant to understanding the migration of cancer cells which migrate to invade other tissues. In addition we will learn about the mechanism by which Wnt signalling controls cell migration.

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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