Award details

Commercial applications of novel enzyme-oligonucleotide conjugates

Reference	BB/C524346/1
Principal Investigator / Supervisor	Professor Keith R Fox
Co-Investigators / Co-Supervisors	Professor Tom Brown
Institution	University of Southampton
Department	Centre for Biological Sciences
Funding type	Research
Value (£)	59,721
Status	Completed
Type	Research Grant
Start date	01/08/2005
End date	31/07/2006
Duration	12 months

Abstract

The potential applications of proteins attached to oligonucleotides is huge, but widespread adoption in fundamental research and industry has been hampered by difficulties in their synthesis. The attachment of proteins to oligonucleotides is usually achieved using chemical cross-linking agents and this suffers from many severe limitations. The aims of this proposal are to exploit a novel methodology to develop a simple, robust and versatile method for linking oligonucleotides to his-tagged proteins. This strategy will be used in the following commercially important areas: 1) Development of novel sequence specific nucleases based on NTA oligonucleotides and His-tagged proteins for use in DNA manipulation; 2) Development of oligonucleotide-protein conjugates for use in detection techniques; 3) Development of the method for generating controlled homo- and hetero-oligomers of proteins; 4) Development of kit formats to enable these technologies to be used routinely in molecular biology laboratories; 5) Development of NTA/FAM and NTA/phenanthroline hybridisation probes in SNP analysis and mutation detection.

Summary

The potential applications of proteins attached to oligonucleotides is huge, but widespread adoption in fundamental research and industry has been hampered by difficulties in their synthesis. The attachment of proteins to oligonucleotides is usually achieved using chemical cross-linking agents and this suffers from many severe limitations. The aims of this proposal are to exploit a novel methodology to develop a simple, robust and versatile method for linking oligonucleotides to his-tagged proteins. This strategy will be used to in the following commercially important areas: · Development of novel sequence specific nucleases based on NTA oligonucleotides and His-tagged proteins for use in DNA manipulation· Development of oligonucleotide-protein conjugates for use in detection techniques.· Development of the method for generating controlled homo- and hetero-oligomers of proteins· Development of kit formats to enable these technologies to be used routinely in molecular biology laboratories · Development of NTA/FAM and NTA/phenanthroline hybridisation probes in SNP analysis and mutation detection

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Follow-On Fund (FOF) [2004-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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