Award details

Sequencing of the Peronospora parasitica genome

Reference	BB/C521244/1
Principal Investigator / Supervisor	Professor Jane Rogers
Co-Investigators / Co-Supervisors
Institution	Wellcome Trust Sanger Institute
Department	Wellcome Trust Sanger Institute Department
Funding type	Research
Value (£)	237,806
Status	Completed
Type	Research Grant
Start date	01/05/2005
End date	30/04/2008
Duration	36 months

Abstract

Peronospora parasitica, downy mildew, is an obligate biotrophic oomyecte pathogen that causes disease on Arabidopsis and Brassica crops. It has been used as a model, naturally occurring, parasite to analyse disease resistance mechanisms in Arabidopsis, leading to the cloning of numerous disease resistance genes and the elucidation of signal transduction pathways. The oomycetes are not fungi and are related to the brown algae and, hence, little is known of their genome structure. Several oomycetes cause major diseases on range of plants such as Phytophthora infestans on potato, P. sojae on soyabean, P. ramorum on tree species, B. lactucae on lettuce and Pe. parasitica and A. candida on Brassica. Hence, Pe. parasitica represents a key linking organism between Arabidopsis and crop plants via the oomycete pathogens. This application is joint between HRI and Brett Tyler and John McDowell (Virginia Tech., USA) to obtain the genome sequence of Pe. parasitica. The US application is to do 8x shotgun sequencing and assembly of the genome. Using HRI's unique BAC library, the UK component is to carry out BAC end sequencing of 8000 clones, complete sequencing of 100 BAC clones, fingerprint the BAC library to create large BAC contigs and to produce 30,000 EST sequences. The HRI component will provide a range of new genomic tools allowing easy access to the Pe, parasitica genome to clone genes of key importance. In addition, all of these resources will be used to complete the genome assembly and to aid in the annotation of the genome. HRI will co-ordinate a community annotation process. All physical resources will be made publicly available, the EST data will be deposited in the BBSRC funded COGEME database and all genome sequence data will be made publically available via VirginiaTech.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	Crop Science, Microbiology, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

BB/C509123/1 Sequencing of the Peronospora parasitica genome

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