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Targeted induced mutation discovery in barley

Reference	BB/C518481/1
Principal Investigator / Supervisor	Professor Robbie Waugh
Co-Investigators / Co-Supervisors	Professor Peter Shewry
Institution	The James Hutton Institute
Department	Genome Dynamics
Funding type	Research
Value (£)	404,656
Status	Completed
Type	Research Grant
Start date	08/08/2005
End date	07/08/2008
Duration	36 months

Abstract

We have developed two large-scale ethyl methanesulfonate (EMS) mutant populations from the barley (Hordeum vulgare L.) cultivar Optic. DNA was isolated from 9,216 M2 plants and assembled into 1,152 eight-plant pools. We developed a mutation scanning approach that combines cleavage of heteroduplexes using the Cel nuclease (Cel I), post cleavage intercalating dye labelling and subsequent detection of cleaved products on a Transgenomic WAVE-HS. We have screened successfully for mutations in five (of five) genes to date. To enhance the screening process by phenotypic enrichment, 12 to 16M3 progeny from each M2 plant were assessed for visible phenotypes and the data entered into a web accessible database (http:bioinf.scri.sari.ac.uk/distilling/distilling.html). We propose to progress the exploitation of these populations to a point where screening is robust, high throughput and cheaper than it is currently. To achieve this we have assembled a consortium of UK scientists who¿s research would benefit considerably from the identification of mutations in their favourite genes. We have also determined that the demand in the international community for access to such a resource is high. Reduction to practice will therefore bring largely technical challenges but coupling these with biological targets will facilitate biological discovery and scientific progress throughout the UK crop science community.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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