Award details

CDR1-mediated peptide signalling in Arabidopsis disease resistance

Reference	BB/C515655/1
Principal Investigator / Supervisor	Professor Chris Lamb
Co-Investigators / Co-Supervisors	Dr Matthieu Chabannes
Institution	John Innes Centre
Department	Disease and Stress Biology
Funding type	Research
Value (£)	240,721
Status	Completed
Type	Research Grant
Start date	01/03/2005
End date	29/02/2008
Duration	36 months

Abstract

The demonstration that CDR1 encodes an apoplastic aspartic protease required for both local and systemic disease resistance responses through the release of a small signal peptide establishes a novel mechanism in plant disease resistance signalling. Complementary strategies will now be developed for identification of the peptide signal released by CDR1 action. Comparison of the apoplast proteome from control and induced tissues should lead to direct identification of candidate peptides. Alternatively, expression of epitope-tagged active site mutants will be used to isolate CDR1 complexes for characterisation of physiological substrates by mass spectrometry and hence delineation of candidate peptides. Experimental protocols initially developed to monitor CDR1 action will be adapted to test candidate peptide signals. Identification of the CDR1-mediated peptide signal, together with phenotypic analysis of CDR1 over-expression and antisense lines crossed with other key signal mutants, will provide the basis for positioning the CDR1 pathways in the overall disease resistance signal network and establishing the physiological properties of the peptide signal system.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search