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Towards the structural determination of multi protein complexes

Reference	BB/C515463/1
Principal Investigator / Supervisor	Professor David Rice
Co-Investigators / Co-Supervisors	Professor Peter Artymiuk, Dr Patrick Baker, Professor Walter Blackstock, Professor Per Bullough, Professor David Hornby, Professor Peter William Piper, Dr John Rafferty, Professor Peter Edwin Sudbery
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	597,160
Status	Completed
Type	Research Grant
Start date	01/06/2005
End date	31/07/2008
Duration	38 months

Abstract

Over the last forty years, investigations of key biological processes have provided insights into the ways in which proteins assemble into large complexes in order to carry out their biochemical functions. One of the most significant realisations arising out of post-genomic science is that the orchestrated interactions of proteins during growth and development is the rule rather than the exception. While the isolation and molecular characterisation of multiprotein complexes is a well recognised area of biological research, new tools and technology driven by the -omic era provide a unique opportunity to learn in unprecedented depth the role of such protein complexes in living organisms. It is the purpose of this application to establish a platform for the development of novel methods for the analysis of multiprotein complexes. Our programme harnesses expertise in yeast genetics and builds on the results of a TAP-tagging survey by Cellzome which has described the repertoire of supramolecular complexes present in this organism. We plan over three years to produce a steady stream of yeast cell pastes, containing different tagged complexes, in quantities sufficient for purification using affinity methods and a novel PhyNexus technology. Subsequently these samples will be subjected to preliminary biochemical and biophysical characterisation to determine the quality and composition of our samples and thereafter nanolitre-scale crystallisation trials and preliminary EM analysis will be carried out.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	Microbiology, Structural Biology, Technology and Methods Development
Research Priority	X – Research Priority information not available
Research Initiative	Structural Proteomics of Rational Targets (SPORT) [2003-2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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