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Interplay between phage lambda and host recombinases in inflation of genetic exchange
Reference
BB/C514523/1
Principal Investigator / Supervisor
Dr Gary John Sharples
Co-Investigators /
Co-Supervisors
Professor Adrian Walmsley
Institution
Durham University
Department
Biological and Biomedical Sciences
Funding type
Research
Value (£)
193,753
Status
Completed
Type
Research Grant
Start date
01/03/2005
End date
29/02/2008
Duration
36 months
Abstract
Genetic exchange in bacteriophages regularly occurs at aberrant sites leading to rearrangement of existing genes or acquisition of new ones. This relaxed fidelity has important consequences for the rate of evolutionary change and the transmission of virulence components between species. We will examine the interplay between a novel DNA binding protein, orf, and recombinases from phage lambda and its host bacterium using stopped-flow fluorescence spectroscopy and electrophoretic mobility shift assays. Loading and unloading of these proteins at the junction between strands and duplexes is likely to be crucial in determining whether annealing or invasion pathways of recombination predominate. The results will offer fresh insights into the mechanisms of recombination responsible for modulating phage genome organisation.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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