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An integrative transcriptome and metabolic profiling study of resource mobilization in wheat

Reference	BB/C514066/1
Principal Investigator / Supervisor	Professor Malcolm Hawkesford
Co-Investigators / Co-Supervisors	Dr Peter Barraclough, Professor Michael Beale, Mr Paul Verrier, Dr Jane Ward
Institution	Rothamsted Research
Department	Plant Biology & Crop Science
Funding type	Research
Value (£)	318,425
Status	Completed
Type	Research Grant
Start date	19/06/2005
End date	18/06/2008
Duration	36 months

Abstract

Nutrient utilization in wheat will be investigated using the 160 years old Broadbalk experiment at Rothamsted and a field trial with varieties selected for contrasting source-sink resource patterns (high protein, high starch, large and small leaf varieties, fast developing and non-dwarfed variety). Trancriptomic and metabolomic data will be generated and combined to generate a co-response network. In this proposed study plant material will be analysed during early grain filling with a focus on leaf tissue and how nutrient-supply affects the capacity and potential to influence grain filling. At this growth stage, the N status of the leaves is a major determinate of the potential for grain filling and redistribution is dependent upon timing and rate of senescence. Photosynthesis providing carbon will decrease concomitantly. Wheat from standard and low N-inputs (50 or 192 kgN ha) as well as a sulphur-deficient plot will be sampled. This will allow the determination of expression profiles in relation to available nutrients, under conditions when yield and nitrogen use efficiently (NUE) varies considerably. The metabolite profiling will help also to determine the metabolic responses to amount and form of N-supply. PCA analysis and clustering analysis will be applied to the individual data sets. Bioinformatics analysis of both data sets will enable responses of networks of genes and metabolites to be determined. The combined response network will help in the determination of previously unidentified key components (regulatory nodes). The newly available Affymetrix wheat chip will be used to profile the transcriptome of the leaves and concurrently metabolite profiling will be achieved by 600MHz LC-NMR-MS, GS-MS and LC-MS at the National Centre for Plant and Microbial Metabolomics, Rothamsted, while other relevant analyses will be carried out in-house total-N-content, elemental analysis by ICP-AES and inorganic ions (HPLC-ion chromatography). This will complement existing transcriptome data from the developing wheat grain which has also demonstrated the utility of sampling from this field experiment.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	Crop Science, Plant Science
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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