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The role of melanophilin in melanosome transport

Reference	BB/C513993/1
Principal Investigator / Supervisor	Professor Miguel Seabra
Co-Investigators / Co-Supervisors	Dr Alistair Hume
Institution	Imperial College London
Department	Div of Biomedical Sciences
Funding type	Research
Value (£)	247,274
Status	Completed
Type	Research Grant
Start date	01/04/2005
End date	31/03/2008
Duration	36 months

Abstract

Melanosome transport is an excellent model system for studying intracellular organelle motility, and the interplay between membrane traffic and the cytoskeleton. We will examine the role of melanophilin, a myosin Va and Rab27 a-interacting protein, in melanosome transport. We will use a melanophilin-null melanocyte cell line to investigate the role of different domains of melanophilin in melanosome transport. We will use these cells to address issues of functional redundancy between melanophilin and the related protein Myrip, and the role of proteins that interact with the C-terminus of melanophilin in melanosome transport. We will use total internal reflectance fluorescence microscopy and other techniques to investigate the role of melanophilin in the transfer of melanosomes from microtubule to actin-based transport systems in real-time. These studies should provide new insights into the function of melanophilin and the molecular mechanisms regulating organelle movement on microtubule and actin-based transport systems.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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